Abstract
Quantifying depth-resolved nanoscale dynamics in biological specimens (e.g., cells) provides a new dimension of study, which may serve as a means of phenotypically differentiating cellular responses to external stimuli and measuring molecular interactions in diverse platforms. Spectral-domain optical coherence phase microscopy (SD-OCPM) represents a novel optical microscopy technique, capable of measuring optical path-length changes inside the specimens with nanometer-level sensitivity. In this chapter, we describe operating principle and implementation of SD-OCPM. Theoretical discussion of SD-OCPM phase stability and spatial resolution will be presented, along with representative applications of SD-OCPM for quantitative biology. Future outlook and potential applications of SD-OCPM will also be discussed.
| Original language | English |
|---|---|
| Title of host publication | Handbook of Photonics for Biomedical Engineering |
| Publisher | Springer Netherlands |
| Pages | 325-350 |
| Number of pages | 26 |
| ISBN (Electronic) | 9789400750524 |
| ISBN (Print) | 9789400750517 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media Dordrecht 2017.
All Science Journal Classification (ASJC) codes
- Engineering(all)
- Physics and Astronomy(all)
- Biochemistry, Genetics and Molecular Biology(all)