Size characterization of core-shell poly(l-lactide) microspheres by flow/hyperlayer field-flow fractionation

Myeong Hee Moon, Keummi Kim, Youngro Byun, Dongjin Pyo

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13 Citations (Scopus)


Flow/hyperlayer field flow fractionation (Fl/Hy FFF) has been applied for the rapid separation and size characterization of core-shell type poly(l- lactide) (PLLA) microspheres which are widely studied as a potential drug carrier due to its biodegradability. Examined microspheres are prepared by varying the entrapped amount of the retinoic acid as an anti-cancer therapeutic and varying PEG-PLLA copolymers. It is demonstrated that the separation of PLLA microspheres can be achieved within 5 min. by Fl/Hy FFF and the particle size distribution (PSD) is readily obtained from the experimental fractograms. From the size distribution of PLLA particles obtained by Fl/Fl FFF, it is shown that PSD increases with the amount of drug loaded but it does not seriously change with the addition of PEG-PLLA copolymers.

Original languageEnglish
Pages (from-to)2729-2740
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Issue number18
Publication statusPublished - 1999

Bibliographical note

Funding Information:
This study was supported by KOSEF Fund 960501-7. Y. B. is grateful for the partial support (Grant HMP96G2-32) from the Korea Ministry of Health & Welfare under the ’96 Highly Advanced National Projects on the Development of Biomedical Engineering & Technology.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry


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