Abstract
We aimed to evaluate the expression of amine oxidase-related proteins in metastatic breast cancer tissue and determine its clinical implication. A tissue microarray was constructed from a total of 126 metastatic breast tumors (31 bone metastases (24.6%), 36 brain metastases (28.6%), 11 liver metastases (8.7%), and 48 lung metastases (38.1%)). Immunohistochemical staining for amine oxidase-related proteins (lysyl oxidase, diamine oxidase, and monoamine oxidase A and B) was performed. In metastatic breast cancer tissue, lysyl oxidase (p = 0.001), tumoral diamine oxidase (p = 0.003), stromal diamine oxidase (p = 0.047), and stromal monoamine oxidase B (p = 0.002) were differentially expressed in different metastatic sites. Bone metastases showed low expression of lysyl oxidase, tumoral diamine oxidase, and stromal diamine oxidase. We observed high expression of lysyl oxidase in brain metastases, tumoral diamine oxidase in liver metastases, stromal diamine oxidase in lung metastases, and stromal monoamine oxidase B in bone metastases. Lysyl oxidase positivity was associated with progesterone receptor negativity (p = 0.001), and monoamine oxidase A positivity was associated with human epidermal growth factor receptor-2 negativity (p = 0.003) and the luminal A subtype (p = 0.003). On univariate analysis shorter overall survival was associated with stromal diamine oxidase negativity (p = 0.008), especially in lung metastases (p = 0.025), and stromal monoamine oxidase B positivity (p < 0.001). Stromal monoamine oxidase B positivity was an independent prognostic factor for shorter overall survival in multivariate Cox analysis (hazard ratio, 4.069; 95% confidence interval, 1.649–10.04; p = 0.002). Finally, in metastatic breast cancer, amine oxidase-related proteins were differentially expressed in a manner specific to metastatic site, and stromal monoamine oxidase B expression was correlated with prognosis.
| Original language | English |
|---|---|
| Journal | Tumor Biology |
| Volume | 40 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2018 May 1 |
Bibliographical note
Funding Information:The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by a Grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, and Republic of Korea (1420080). This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A1A1A05001209).
Funding Information:
C.Y.J. participated in the design of the study, performed statistical analysis, and carried out the immunoassays. J.W.H. participated in its design. J.S.K. conceived the study, participated in its design and coordination, and helped to draft the manuscript. All the authors read and approved the final manuscript. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by a Grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, and Republic of Korea (1420080). This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A1A1A05001209).
Publisher Copyright:
© 2018, © The Author(s) 2018.
All Science Journal Classification (ASJC) codes
- Cancer Research
