TY - JOUR
T1 - Site-directed mutagenesis of a loop at the active site of E1 (α2β2) of the pyruvate dehydrogenase complex
T2 - A possible common sequence motif
AU - Fries, Markus
AU - Chauhan, Hitesh J.
AU - Domingo, Gonzalo J.
AU - Jung, Hyo Il
AU - Perham, Richard N.
PY - 2003/3
Y1 - 2003/3
N2 - Limited proteolysis of the pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase (PDH) multi-enzyme complex of Bacillus stearothermophilus has indicated the importance for catalysis of a site (Tyr281-Arg282) in the E1α subunit (Chauhan, H.J., Domingo, G.J., Jung, H.-I. & Perham, R.N. (2000) Eur. J. Biochem. 267, 7158-7169). This site appears to be conserved in the α-subunit of heterotetrameric E1s and multiple sequence alignments suggest that there are additional conserved amino-acid residues in this region, part of a common pattern with the consensus sequence -YR-H-D-YR-DE-. This region lies about 50 amino acids on the C-terminal side of a 30-residue motif previously recognized as involved in binding thiamin diphosphate (ThDP) in all ThDP-dependent enzymes. The role of individual residues in this set of conserved amino acids in the E1α chain was investigated by means of site-directed mutagenesis. We propose that particular residues are involved in: (a) binding the 2-oxo acid substrate, (b) decarboxylation of the 2-oxo acid and reductive acetylation of the tethered lipoyl domain in the PDH complex, (c) an 'open-close' mechanism of the active site, and (d) phosphorylation by the E1-specific kinase (in eukaryotic PDH and branched chain 2-oxo acid dehydrogenase complexes).
AB - Limited proteolysis of the pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase (PDH) multi-enzyme complex of Bacillus stearothermophilus has indicated the importance for catalysis of a site (Tyr281-Arg282) in the E1α subunit (Chauhan, H.J., Domingo, G.J., Jung, H.-I. & Perham, R.N. (2000) Eur. J. Biochem. 267, 7158-7169). This site appears to be conserved in the α-subunit of heterotetrameric E1s and multiple sequence alignments suggest that there are additional conserved amino-acid residues in this region, part of a common pattern with the consensus sequence -YR-H-D-YR-DE-. This region lies about 50 amino acids on the C-terminal side of a 30-residue motif previously recognized as involved in binding thiamin diphosphate (ThDP) in all ThDP-dependent enzymes. The role of individual residues in this set of conserved amino acids in the E1α chain was investigated by means of site-directed mutagenesis. We propose that particular residues are involved in: (a) binding the 2-oxo acid substrate, (b) decarboxylation of the 2-oxo acid and reductive acetylation of the tethered lipoyl domain in the PDH complex, (c) an 'open-close' mechanism of the active site, and (d) phosphorylation by the E1-specific kinase (in eukaryotic PDH and branched chain 2-oxo acid dehydrogenase complexes).
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U2 - 10.1046/j.1432-1033.2003.03444.x
DO - 10.1046/j.1432-1033.2003.03444.x
M3 - Article
C2 - 12603319
AN - SCOPUS:0037338412
SN - 0014-2956
VL - 270
SP - 861
EP - 870
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 5
ER -