Single-strand annealing mediates the conservative repair of double-strand DNA breaks in homologous recombination-defective germ cells of Caenorhabditis elegans

Woori Bae; Seokbong Hong; Mi So Park; Ha Kyeong Jeong; Myon Hee Lee; Hyeon Sook Koo

doi:10.1016/j.dnarep.2019.01.007

Single-strand annealing mediates the conservative repair of double-strand DNA breaks in homologous recombination-defective germ cells of Caenorhabditis elegans

Woori Bae, Seokbong Hong, Mi So Park, Ha Kyeong Jeong, Myon Hee Lee, Hyeon Sook Koo

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A missense mutation in C. elegans RAD-54, a homolog of RAD54 that operates in the homologous recombination (HR) pathway, was found to decrease ATPase activity in vitro. The hypomorphic mutation caused hypersensitivity of C. elegans germ cells to double-strand DNA breaks (DSBs). Although the formation of RAD-51 foci at DSBs was normal in both the mutant and knockdown worms, their subsequent dissipation was slow. The rad-54-deficient phenotypes were greatly aggravated when combined with an xpf-1 mutation, suggesting a conservative role of single-strand annealing (SSA) for DSB repair in HR-defective worms. The phenotypes of doubly-deficient rad-54;xpf-1 worms were partially suppressed by a mutation of lig-4, a nonhomologous end-joining (NHEJ) factor. In summary, RAD-54 is required for the dissociation of RAD-51 from DSB sites in C. elegans germ cells. Also, NHEJ and SSA exert negative and positive effects, respectively, on genome stability when HR is defective.

Original language	English
Pages (from-to)	18-28
Number of pages	11
Journal	DNA Repair
Volume	75
DOIs	https://doi.org/10.1016/j.dnarep.2019.01.007
Publication status	Published - 2019 Mar

Bibliographical note

Funding Information:
This work was supported by The National Research Foundation of Korea [the Basic Science Research program, 2016R1D1A1B03934743 to H.-S.K.]; the Brain Korea 21 ( BK21 ) PLUS program.

Funding Information:
The wild-type Bristol N2, lig-4(ok716), xpa-1(ok698), and VC20429 [containing rad-54(gk118019) allele] strains were acquired from the C. elegans Genetics Center (St Paul, MN, USA), which is funded by the NIH Office of Research Infrastructure Programs ( P40 OD010440 ). We thank Dr. Shohei Mitani (Tokyo Women’s Medical University School of Medicine) for the xpf-1(tm2842), xpc-1(tm3886), and polq-1(tm2572) mutants, and Dr. Hannes Lans (Erasmus Medical Center, Netherlands) for sending outcrossed ercc-1(tm2073) worms. We also thank Dr. George Chung (University of British Columbia, Canada) for reading this manuscript and providing helpful comments. We very much appreciated the technical assistance of Sin-Ae Oh (Yonsei University, Republic of Korea).

Publisher Copyright:
© 2019 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.dnarep.2019.01.007

Cite this

@article{562fccef89c642d6a7161cf272ccc001,

title = "Single-strand annealing mediates the conservative repair of double-strand DNA breaks in homologous recombination-defective germ cells of Caenorhabditis elegans",

abstract = "A missense mutation in C. elegans RAD-54, a homolog of RAD54 that operates in the homologous recombination (HR) pathway, was found to decrease ATPase activity in vitro. The hypomorphic mutation caused hypersensitivity of C. elegans germ cells to double-strand DNA breaks (DSBs). Although the formation of RAD-51 foci at DSBs was normal in both the mutant and knockdown worms, their subsequent dissipation was slow. The rad-54-deficient phenotypes were greatly aggravated when combined with an xpf-1 mutation, suggesting a conservative role of single-strand annealing (SSA) for DSB repair in HR-defective worms. The phenotypes of doubly-deficient rad-54;xpf-1 worms were partially suppressed by a mutation of lig-4, a nonhomologous end-joining (NHEJ) factor. In summary, RAD-54 is required for the dissociation of RAD-51 from DSB sites in C. elegans germ cells. Also, NHEJ and SSA exert negative and positive effects, respectively, on genome stability when HR is defective.",

author = "Woori Bae and Seokbong Hong and Park, {Mi So} and Jeong, {Ha Kyeong} and Lee, {Myon Hee} and Koo, {Hyeon Sook}",

note = "Funding Information: This work was supported by The National Research Foundation of Korea [the Basic Science Research program, 2016R1D1A1B03934743 to H.-S.K.]; the Brain Korea 21 ( BK21 ) PLUS program. Funding Information: The wild-type Bristol N2, lig-4(ok716), xpa-1(ok698), and VC20429 [containing rad-54(gk118019) allele] strains were acquired from the C. elegans Genetics Center (St Paul, MN, USA), which is funded by the NIH Office of Research Infrastructure Programs ( P40 OD010440 ). We thank Dr. Shohei Mitani (Tokyo Women{\textquoteright}s Medical University School of Medicine) for the xpf-1(tm2842), xpc-1(tm3886), and polq-1(tm2572) mutants, and Dr. Hannes Lans (Erasmus Medical Center, Netherlands) for sending outcrossed ercc-1(tm2073) worms. We also thank Dr. George Chung (University of British Columbia, Canada) for reading this manuscript and providing helpful comments. We very much appreciated the technical assistance of Sin-Ae Oh (Yonsei University, Republic of Korea). Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = mar,

doi = "10.1016/j.dnarep.2019.01.007",

language = "English",

volume = "75",

pages = "18--28",

journal = "DNA Repair",

issn = "1568-7864",

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T1 - Single-strand annealing mediates the conservative repair of double-strand DNA breaks in homologous recombination-defective germ cells of Caenorhabditis elegans

AU - Bae, Woori

AU - Hong, Seokbong

AU - Park, Mi So

AU - Jeong, Ha Kyeong

AU - Lee, Myon Hee

AU - Koo, Hyeon Sook

N1 - Funding Information: This work was supported by The National Research Foundation of Korea [the Basic Science Research program, 2016R1D1A1B03934743 to H.-S.K.]; the Brain Korea 21 ( BK21 ) PLUS program. Funding Information: The wild-type Bristol N2, lig-4(ok716), xpa-1(ok698), and VC20429 [containing rad-54(gk118019) allele] strains were acquired from the C. elegans Genetics Center (St Paul, MN, USA), which is funded by the NIH Office of Research Infrastructure Programs ( P40 OD010440 ). We thank Dr. Shohei Mitani (Tokyo Women’s Medical University School of Medicine) for the xpf-1(tm2842), xpc-1(tm3886), and polq-1(tm2572) mutants, and Dr. Hannes Lans (Erasmus Medical Center, Netherlands) for sending outcrossed ercc-1(tm2073) worms. We also thank Dr. George Chung (University of British Columbia, Canada) for reading this manuscript and providing helpful comments. We very much appreciated the technical assistance of Sin-Ae Oh (Yonsei University, Republic of Korea). Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/3

Y1 - 2019/3

N2 - A missense mutation in C. elegans RAD-54, a homolog of RAD54 that operates in the homologous recombination (HR) pathway, was found to decrease ATPase activity in vitro. The hypomorphic mutation caused hypersensitivity of C. elegans germ cells to double-strand DNA breaks (DSBs). Although the formation of RAD-51 foci at DSBs was normal in both the mutant and knockdown worms, their subsequent dissipation was slow. The rad-54-deficient phenotypes were greatly aggravated when combined with an xpf-1 mutation, suggesting a conservative role of single-strand annealing (SSA) for DSB repair in HR-defective worms. The phenotypes of doubly-deficient rad-54;xpf-1 worms were partially suppressed by a mutation of lig-4, a nonhomologous end-joining (NHEJ) factor. In summary, RAD-54 is required for the dissociation of RAD-51 from DSB sites in C. elegans germ cells. Also, NHEJ and SSA exert negative and positive effects, respectively, on genome stability when HR is defective.

AB - A missense mutation in C. elegans RAD-54, a homolog of RAD54 that operates in the homologous recombination (HR) pathway, was found to decrease ATPase activity in vitro. The hypomorphic mutation caused hypersensitivity of C. elegans germ cells to double-strand DNA breaks (DSBs). Although the formation of RAD-51 foci at DSBs was normal in both the mutant and knockdown worms, their subsequent dissipation was slow. The rad-54-deficient phenotypes were greatly aggravated when combined with an xpf-1 mutation, suggesting a conservative role of single-strand annealing (SSA) for DSB repair in HR-defective worms. The phenotypes of doubly-deficient rad-54;xpf-1 worms were partially suppressed by a mutation of lig-4, a nonhomologous end-joining (NHEJ) factor. In summary, RAD-54 is required for the dissociation of RAD-51 from DSB sites in C. elegans germ cells. Also, NHEJ and SSA exert negative and positive effects, respectively, on genome stability when HR is defective.

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DO - 10.1016/j.dnarep.2019.01.007

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AN - SCOPUS:85060694570

SN - 1568-7864

VL - 75

SP - 18

EP - 28

JO - DNA Repair

JF - DNA Repair

ER -

Single-strand annealing mediates the conservative repair of double-strand DNA breaks in homologous recombination-defective germ cells of Caenorhabditis elegans

Abstract

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