Single-exposure quantitative phase imaging in color-coded LED microscopy

Wonchan Lee, Daeseong Jung, Suho Ryu, Chulmin Joo

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with equal area, assigned to red, green, and blue colors. Image acquisition with a color image sensor and subsequent computation based on weak object transfer functions allow for the QPI of a transparent specimen. We also provide a correction method for color-leakage, which may be encountered in implementing our method with consumer-grade LEDs and image sensors. Most commercially available LEDs and image sensors do not provide spectrally isolated emissions and pixel responses, generating significant error in phase estimation in our method. We describe the correction scheme for this color-leakage issue, and demonstrate improved phase measurement accuracy. The computational model and single-exposure QPI capability of our method are presented by showing images of calibrated phase samples and cellular specimens.

Original languageEnglish
Pages (from-to)8398-8411
Number of pages14
JournalOptics Express
Issue number7
Publication statusPublished - 2017 Apr 3

Bibliographical note

Publisher Copyright:
© 2017 Optical Society of America.

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics


Dive into the research topics of 'Single-exposure quantitative phase imaging in color-coded LED microscopy'. Together they form a unique fingerprint.

Cite this