TY - GEN
T1 - Single-cell transcriptome analysis to study tumor-stromal interaction on cancer-stem-cells using vertical adhesion-suspension co-culture platforms
AU - Chen, Yu Chih
AU - Zhang, Zhixiong
AU - Cheng, Yu Heng
AU - Yoon, Euisik
PY - 2016
Y1 - 2016
N2 - The interaction between cancer-stem-cells and stromal cells is critical for development of tumor. In this work, we have developed a high-throughput (10,000-well), user-friendly, vertical co-culture platform for tumorsphere assays in interaction with cancer-associated-fibroblasts (CAFs). This platform has improved the throughput by more than two orders of magnitude. In addition, single-cell transcriptome analysis (mRNA Next Generation Sequencing (NGS)) was performed on the retrieved single cells, providing insights into the nature of functional CSCs.
AB - The interaction between cancer-stem-cells and stromal cells is critical for development of tumor. In this work, we have developed a high-throughput (10,000-well), user-friendly, vertical co-culture platform for tumorsphere assays in interaction with cancer-associated-fibroblasts (CAFs). This platform has improved the throughput by more than two orders of magnitude. In addition, single-cell transcriptome analysis (mRNA Next Generation Sequencing (NGS)) was performed on the retrieved single cells, providing insights into the nature of functional CSCs.
UR - http://www.scopus.com/inward/record.url?scp=85014198949&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014198949&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014198949
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 71
EP - 72
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -
