Single-cell cytokine profiling to investigate cellular functional diversity in hematopoietic malignancies

Jonathan J. Chen; Minsuk Kwak; Rong Fan

doi:10.1007/978-1-4939-4011-0_19

Single-cell cytokine profiling to investigate cellular functional diversity in hematopoietic malignancies

Jonathan J. Chen, Minsuk Kwak, Rong Fan

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a highdensity antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	243-254
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-4939-4011-0_19
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1465
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-4011-0_19

Cite this

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abstract = "Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a highdensity antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations.",

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Single-cell cytokine profiling to investigate cellular functional diversity in hematopoietic malignancies

Abstract

Publication series

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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