Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers; T. K. Lake; L. Paterson; J. W. Allen; W. Sibbett; K. Dholakia

doi:10.1109/CLEOE.2003.1313780

Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers, T. K. Lake, L. Paterson, J. W. Allen, W. Sibbett, K. Dholakia

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

Original language	English
Title of host publication	2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003
Pages	712
Number of pages	1
DOIs	https://doi.org/10.1109/CLEOE.2003.1313780
Publication status	Published - 2003
Event	2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003 - Munich, Germany Duration: 2003 Jun 22 → 2003 Jun 27

Publication series

Name	Conference on Lasers and Electro-Optics Europe - Technical Digest

Conference

Conference	2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003
Country/Territory	Germany
City	Munich
Period	03/6/22 → 03/6/27

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials

Access to Document

10.1109/CLEOE.2003.1313780

Cite this

Carruthers, A. E., Lake, T. K., Paterson, L., Allen, J. W., Sibbett, W., & Dholakia, K. (2003). Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. In 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003 (pp. 712). Article 1313780 (Conference on Lasers and Electro-Optics Europe - Technical Digest). https://doi.org/10.1109/CLEOE.2003.1313780

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title = "Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes",

abstract = "In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.",

author = "Carruthers, {A. E.} and Lake, {T. K.} and L. Paterson and Allen, {J. W.} and W. Sibbett and K. Dholakia",

year = "2003",

doi = "10.1109/CLEOE.2003.1313780",

language = "English",

isbn = "0780377346",

series = "Conference on Lasers and Electro-Optics Europe - Technical Digest",

pages = "712",

booktitle = "2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003",

note = "2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003 ; Conference date: 22-06-2003 Through 27-06-2003",

}

Carruthers, AE, Lake, TK, Paterson, L, Allen, JW, Sibbett, W & Dholakia, K 2003, Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. in 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003., 1313780, Conference on Lasers and Electro-Optics Europe - Technical Digest, pp. 712, 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003, Munich, Germany, 03/6/22. https://doi.org/10.1109/CLEOE.2003.1313780

Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. / Carruthers, A. E.; Lake, T. K.; Paterson, L. et al.
2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003. 2003. p. 712 1313780 (Conference on Lasers and Electro-Optics Europe - Technical Digest).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

AU - Carruthers, A. E.

AU - Lake, T. K.

AU - Paterson, L.

AU - Allen, J. W.

AU - Sibbett, W.

AU - Dholakia, K.

PY - 2003

Y1 - 2003

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AB - In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

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U2 - 10.1109/CLEOE.2003.1313780

DO - 10.1109/CLEOE.2003.1313780

M3 - Conference contribution

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SN - 9780780377349

T3 - Conference on Lasers and Electro-Optics Europe - Technical Digest

SP - 712

BT - 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003

T2 - 2003 Conference on Lasers and Electro-Optics Europe, CLEO/EUROPE 2003

Y2 - 22 June 2003 through 27 June 2003

ER -

Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

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All Science Journal Classification (ASJC) codes

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