TY - JOUR
T1 - Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes
AU - Kinsky, Nathaniel R.
AU - Vöröslakos, Mihály
AU - Lopez Ruiz, Jose Roberto
AU - Watkins de Jong, Laurel
AU - Slager, Nathan
AU - McKenzie, Sam
AU - Yoon, Euisik
AU - Diba, Kamran
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/12/15
Y1 - 2023/12/15
N2 - Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment. For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1
AB - Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment. For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1
KW - Biotechnology and Bioengineering
KW - Neuroscience
KW - Systems Biology
UR - http://www.scopus.com/inward/record.url?scp=85171283995&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85171283995&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2023.102570
DO - 10.1016/j.xpro.2023.102570
M3 - Article
C2 - 37729059
AN - SCOPUS:85171283995
SN - 2666-1667
VL - 4
JO - STAR Protocols
JF - STAR Protocols
IS - 4
M1 - 102570
ER -