Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa

Roberta Migliavacca, Jean Denis Docquier, Claudia Mugnaioli, Gianfranco Amicosante, Rossana Daturi, Kyungwon Lee, Gian Maria Rossolini, Laura Pagani

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM-and 4 IMP-type metallo-β-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.

Original languageEnglish
Pages (from-to)4388-4390
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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