Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana

Jong A. Park; Tae Wuk Kim; Seong Ki Kim; Taek Kim Woo; Hyun Sook Pai

doi:10.1016/j.febslet.2005.07.013

Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana

Jong A. Park, Tae Wuk Kim, Seong Ki Kim, Taek Kim Woo, Hyun Sook Pai

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Abstract

The very long chain fatty acids (VLCFAs) are synthesized by the microsomal fatty acid elongation system in plants. We investigated cellular function of NbECR putatively encoding enoyl-CoA reductase that catalyzes the last step of VLCFA elongation in Nicotiana benthamiana. Virus-induced gene silencing of NbECR produced necrotic lesions with typical cell death symptoms in leaves. In the affected tissues, ablation of the epidermal cell layer preceded disintegration of the whole leaf cell layers, and disorganized cellular membrane structure was evident. The amount of VLCFAs was reduced in the NbECR VIGS lines, suggesting NbECR function in elongation of VLCFAs. The results demonstrate that NbECR encodes a putative enoyl-CoA reductase and that the NbECR activity is essential for membrane biogenesis in N. benthamiana.

Original language	English
Pages (from-to)	4459-4464
Number of pages	6
Journal	FEBS Letters
Volume	579
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2005.07.013
Publication status	Published - 2005 Aug 15

Bibliographical note

Funding Information:
This research was supported by grants from the Plant Diversity Research Center of the 21st Century Frontier Research Program (to H.-S. Pai and W.T. Kim), the Molecular and Cellular BioDiscovery Research Program (to H.-S. Pai), and the Plant Metabolism Research Center (at Kyung Hee University) of the Science Research Center Program (to W.T. Kim), all of which are funded by the Ministry of Science and Technology of the Korean government.

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana",

abstract = "The very long chain fatty acids (VLCFAs) are synthesized by the microsomal fatty acid elongation system in plants. We investigated cellular function of NbECR putatively encoding enoyl-CoA reductase that catalyzes the last step of VLCFA elongation in Nicotiana benthamiana. Virus-induced gene silencing of NbECR produced necrotic lesions with typical cell death symptoms in leaves. In the affected tissues, ablation of the epidermal cell layer preceded disintegration of the whole leaf cell layers, and disorganized cellular membrane structure was evident. The amount of VLCFAs was reduced in the NbECR VIGS lines, suggesting NbECR function in elongation of VLCFAs. The results demonstrate that NbECR encodes a putative enoyl-CoA reductase and that the NbECR activity is essential for membrane biogenesis in N. benthamiana.",

author = "Park, {Jong A.} and Kim, {Tae Wuk} and Kim, {Seong Ki} and Woo, {Taek Kim} and Pai, {Hyun Sook}",

note = "Funding Information: This research was supported by grants from the Plant Diversity Research Center of the 21st Century Frontier Research Program (to H.-S. Pai and W.T. Kim), the Molecular and Cellular BioDiscovery Research Program (to H.-S. Pai), and the Plant Metabolism Research Center (at Kyung Hee University) of the Science Research Center Program (to W.T. Kim), all of which are funded by the Ministry of Science and Technology of the Korean government. ",

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AU - Woo, Taek Kim

AU - Pai, Hyun Sook

N1 - Funding Information: This research was supported by grants from the Plant Diversity Research Center of the 21st Century Frontier Research Program (to H.-S. Pai and W.T. Kim), the Molecular and Cellular BioDiscovery Research Program (to H.-S. Pai), and the Plant Metabolism Research Center (at Kyung Hee University) of the Science Research Center Program (to W.T. Kim), all of which are funded by the Ministry of Science and Technology of the Korean government.

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N2 - The very long chain fatty acids (VLCFAs) are synthesized by the microsomal fatty acid elongation system in plants. We investigated cellular function of NbECR putatively encoding enoyl-CoA reductase that catalyzes the last step of VLCFA elongation in Nicotiana benthamiana. Virus-induced gene silencing of NbECR produced necrotic lesions with typical cell death symptoms in leaves. In the affected tissues, ablation of the epidermal cell layer preceded disintegration of the whole leaf cell layers, and disorganized cellular membrane structure was evident. The amount of VLCFAs was reduced in the NbECR VIGS lines, suggesting NbECR function in elongation of VLCFAs. The results demonstrate that NbECR encodes a putative enoyl-CoA reductase and that the NbECR activity is essential for membrane biogenesis in N. benthamiana.

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Silencing of NbECR encoding a putative enoyl-CoA reductase results in disorganized membrane structures and epidermal cell ablation in Nicotiana benthamiana

Abstract

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