'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules

Hwangbeom Kim, Hyojun Han, Jinwoo Ahn, Joongoo Lee, Namjin Cho, Hoon Jang, Hyoki Kim, Sunghoon Kwon, Duhee Bang

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.

Original languageEnglish
Pages (from-to)e140
JournalNucleic acids research
Issue number18
Publication statusPublished - 2012 Oct

Bibliographical note

Funding Information:
This work was supported by the Intelligent Synthetic Biology Center of the Global Frontier Project funded by the Ministry of Education, Science and Technology, Korea [2011-0031956]. Hwangbeom Kim was supported by a National Junior Research Fellowship, National Research Foundation of Korea [2012-002388]. Funding for open access charge: Ministry of Education, Science and Technology [2011-0031956].

All Science Journal Classification (ASJC) codes

  • Genetics


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