Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay

This paper proposes a new microfluidic array which can sequentially load different types of reagents into a two-dimensional microwell array for high-throughput single cell assay. Proposed array is composed of more than 200 chambers and each sub-array can be selected by microfluidic logics for row and column selection. By applying pneumatic pressure and injecting a different set of reagents, we could load different reagents into the two-dimensional array. In order to verify the sequential addressing scheme, we fabricated and tested a 3x3 device for 9 different reagent loading demonstrated by various colors in food dye.