TY - JOUR
T1 - Separation of Proteins Mixture in Hollow Fiber Flow Field-Flow Fractionation
AU - Shin, Se Jong
AU - Nam, Hyun Hee
AU - Min, Byoung Ryul
AU - Park, Jin Won
AU - An, Ik Sung
AU - Lee, Kangtaek
PY - 2003/9/20
Y1 - 2003/9/20
N2 - Flow field-flow fractionation (FlFFF) is a technology to separate the molecules by size in an open channel. Molecules with different size have different diffusivities and are located vertically in different positions when passing through an open channel. In this study, hollow fiber membranes instead of conventional rectangular channels have been used as materials for the open channel and this change would decrease the cost of manufacturing. FlFFF is a useful technique to characterize the biopolymeric materials. Retention time, diffusion coefficients and Stokes radius of analysis can be calculated from the related simple equations. Hollow-fiber flow field-flow fractionation (HF-FlFFF) has been used for the characterization and separation of protein mixture in a phosphate buffer solution and has demonstrated the potential to be developed into a disposable FIFFF channel. The important indexes for the analytical separation are selectivity, resolution and plate height. The optimized separation condition for protein mixture of Ovalbumin, Alcohol dehydrogenase, Apoferritin and Thyroglobulin is Vout/Vrad = 0.65/0.85 mL/min.
AB - Flow field-flow fractionation (FlFFF) is a technology to separate the molecules by size in an open channel. Molecules with different size have different diffusivities and are located vertically in different positions when passing through an open channel. In this study, hollow fiber membranes instead of conventional rectangular channels have been used as materials for the open channel and this change would decrease the cost of manufacturing. FlFFF is a useful technique to characterize the biopolymeric materials. Retention time, diffusion coefficients and Stokes radius of analysis can be calculated from the related simple equations. Hollow-fiber flow field-flow fractionation (HF-FlFFF) has been used for the characterization and separation of protein mixture in a phosphate buffer solution and has demonstrated the potential to be developed into a disposable FIFFF channel. The important indexes for the analytical separation are selectivity, resolution and plate height. The optimized separation condition for protein mixture of Ovalbumin, Alcohol dehydrogenase, Apoferritin and Thyroglobulin is Vout/Vrad = 0.65/0.85 mL/min.
UR - http://www.scopus.com/inward/record.url?scp=0347692878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347692878&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2003.24.9.1339
DO - 10.5012/bkcs.2003.24.9.1339
M3 - Article
AN - SCOPUS:0347692878
SN - 0253-2964
VL - 24
SP - 1339
EP - 1344
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 9
ER -