A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 μm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.
Bibliographical noteFunding Information:
Acknowledgements This work was supported by the Ministry of Information and Communication, Republic of Korea, under project No. A1100-0501-0073 and a grant from National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation. We also acknowledge support from the Seoul Research and Business Development Program (10816). We are very grateful to Dr. B. Davletov for his kind gift of the C2A protein gene.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology