Separation of apoptotic cells using a microfluidic device

Hyun Seok Kim; Oh Taek Son; Kun Hong Kim; Sang Hyeob Kim; Sunglyul Maeng; Hyo Il Jung

doi:10.1007/s10529-007-9451-1

Separation of apoptotic cells using a microfluidic device

Hyun Seok Kim, Oh Taek Son, Kun Hong Kim, Sang Hyeob Kim, Sunglyul Maeng, Hyo Il Jung

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 μm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.

Original language	English
Pages (from-to)	1659-1663
Number of pages	5
Journal	Biotechnology Letters
Volume	29
Issue number	11
DOIs	https://doi.org/10.1007/s10529-007-9451-1
Publication status	Published - 2007 Nov

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the Ministry of Information and Communication, Republic of Korea, under project No. A1100-0501-0073 and a grant from National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation. We also acknowledge support from the Seoul Research and Business Development Program (10816). We are very grateful to Dr. B. Davletov for his kind gift of the C2A protein gene.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-007-9451-1

Cite this

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title = "Separation of apoptotic cells using a microfluidic device",

abstract = "A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 μm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.",

author = "Kim, {Hyun Seok} and Son, {Oh Taek} and Kim, {Kun Hong} and Kim, {Sang Hyeob} and Sunglyul Maeng and Jung, {Hyo Il}",

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AU - Maeng, Sunglyul

AU - Jung, Hyo Il

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Separation of apoptotic cells using a microfluidic device

Abstract

Bibliographical note

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