Sensing of uric acid via cascade catalysis of uricase and a biomimetic catalyst

Min Chul Kim; Jinyoung Kwak; Sang Yup Lee

doi:10.1016/j.snb.2016.04.033

Sensing of uric acid via cascade catalysis of uricase and a biomimetic catalyst

Min Chul Kim, Jinyoung Kwak, Sang Yup Lee

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A uric acid (UA) sensing system that exploits the cascade catalysis of a natural enzyme and a biomimetic catalyst was developed. In the cascade catalysis system, UA was oxidized by uricase (UOx) to release hydrogen peroxide (H₂O₂), which was further used as a substrate for the oxidation of o-phenylenediamine (OPD) by a biomimetic peroxidase-like catalyst (PMOx). OPD oxidation resulted in a change in color of the UA solution, which enabled quantification of UA through optical spectroscopy. The cascade catalysis system exhibited sensitivity for UA at concentrations greater than 0.01 mM, with linear correlation to the optical intensity. Furthermore, the cascade catalysis system exhibited high selectivity toward UA because of the inherent selectivity of UOx. The cascade catalysis system showed maximum activity at 35 °C because of the deactivation of UOx at higher temperatures, although the PMOx exhibited better catalytic activity with increasing temperature. The sensing of UA in serum samples was examined using the developed system. The sensitivity of this cascade catalysis system was comparable to that of a clinical method, with 5.45% deviation. The combinational use of a natural enzyme and a biomimetic catalyst demonstrated in this study may be further applicable for the development of other biological sensors with advantages in thermal stability and production cost.

Original language	English
Pages (from-to)	744-749
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	232
DOIs	https://doi.org/10.1016/j.snb.2016.04.033
Publication status	Published - 2016 Sept 1

Bibliographical note

Funding Information:
This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ). We appreciate the assistance of Dr. E. J. Lee at Severance Hospital for the serum sample analysis.

Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2016.04.033

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title = "Sensing of uric acid via cascade catalysis of uricase and a biomimetic catalyst",

abstract = "A uric acid (UA) sensing system that exploits the cascade catalysis of a natural enzyme and a biomimetic catalyst was developed. In the cascade catalysis system, UA was oxidized by uricase (UOx) to release hydrogen peroxide (H2O2), which was further used as a substrate for the oxidation of o-phenylenediamine (OPD) by a biomimetic peroxidase-like catalyst (PMOx). OPD oxidation resulted in a change in color of the UA solution, which enabled quantification of UA through optical spectroscopy. The cascade catalysis system exhibited sensitivity for UA at concentrations greater than 0.01 mM, with linear correlation to the optical intensity. Furthermore, the cascade catalysis system exhibited high selectivity toward UA because of the inherent selectivity of UOx. The cascade catalysis system showed maximum activity at 35 °C because of the deactivation of UOx at higher temperatures, although the PMOx exhibited better catalytic activity with increasing temperature. The sensing of UA in serum samples was examined using the developed system. The sensitivity of this cascade catalysis system was comparable to that of a clinical method, with 5.45% deviation. The combinational use of a natural enzyme and a biomimetic catalyst demonstrated in this study may be further applicable for the development of other biological sensors with advantages in thermal stability and production cost.",

author = "Kim, {Min Chul} and Jinyoung Kwak and Lee, {Sang Yup}",

note = "Funding Information: This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ). We appreciate the assistance of Dr. E. J. Lee at Severance Hospital for the serum sample analysis. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V. All rights reserved.",

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N1 - Funding Information: This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ). We appreciate the assistance of Dr. E. J. Lee at Severance Hospital for the serum sample analysis. Publisher Copyright: © 2016 Elsevier B.V. All rights reserved.

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N2 - A uric acid (UA) sensing system that exploits the cascade catalysis of a natural enzyme and a biomimetic catalyst was developed. In the cascade catalysis system, UA was oxidized by uricase (UOx) to release hydrogen peroxide (H2O2), which was further used as a substrate for the oxidation of o-phenylenediamine (OPD) by a biomimetic peroxidase-like catalyst (PMOx). OPD oxidation resulted in a change in color of the UA solution, which enabled quantification of UA through optical spectroscopy. The cascade catalysis system exhibited sensitivity for UA at concentrations greater than 0.01 mM, with linear correlation to the optical intensity. Furthermore, the cascade catalysis system exhibited high selectivity toward UA because of the inherent selectivity of UOx. The cascade catalysis system showed maximum activity at 35 °C because of the deactivation of UOx at higher temperatures, although the PMOx exhibited better catalytic activity with increasing temperature. The sensing of UA in serum samples was examined using the developed system. The sensitivity of this cascade catalysis system was comparable to that of a clinical method, with 5.45% deviation. The combinational use of a natural enzyme and a biomimetic catalyst demonstrated in this study may be further applicable for the development of other biological sensors with advantages in thermal stability and production cost.

AB - A uric acid (UA) sensing system that exploits the cascade catalysis of a natural enzyme and a biomimetic catalyst was developed. In the cascade catalysis system, UA was oxidized by uricase (UOx) to release hydrogen peroxide (H2O2), which was further used as a substrate for the oxidation of o-phenylenediamine (OPD) by a biomimetic peroxidase-like catalyst (PMOx). OPD oxidation resulted in a change in color of the UA solution, which enabled quantification of UA through optical spectroscopy. The cascade catalysis system exhibited sensitivity for UA at concentrations greater than 0.01 mM, with linear correlation to the optical intensity. Furthermore, the cascade catalysis system exhibited high selectivity toward UA because of the inherent selectivity of UOx. The cascade catalysis system showed maximum activity at 35 °C because of the deactivation of UOx at higher temperatures, although the PMOx exhibited better catalytic activity with increasing temperature. The sensing of UA in serum samples was examined using the developed system. The sensitivity of this cascade catalysis system was comparable to that of a clinical method, with 5.45% deviation. The combinational use of a natural enzyme and a biomimetic catalyst demonstrated in this study may be further applicable for the development of other biological sensors with advantages in thermal stability and production cost.

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Sensing of uric acid via cascade catalysis of uricase and a biomimetic catalyst

Abstract

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