Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: Noncovalent strategy in developing a gene delivery carrier

Yong Beom Lim; Taeil Kim; Jae Wook Lee; Seon Mi Kim; Hee Joon Kim; Kimoon Kim; Jong Sang Park

doi:10.1021/bc025581r

Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: Noncovalent strategy in developing a gene delivery carrier

Yong Beom Lim, Taeil Kim, Jae Wook Lee, Seon Mi Kim, Hee Joon Kim, Kimoon Kim, Jong Sang Park

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH₂NH₂⁺(CH₂) ₄NH₃⁺]_z], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.

Original language	English
Pages (from-to)	1181-1185
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	13
Issue number	6
DOIs	https://doi.org/10.1021/bc025581r
Publication status	Published - 2002

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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title = "Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: Noncovalent strategy in developing a gene delivery carrier",

abstract = "A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH2NH2+(CH2) 4NH3+]z], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.",

author = "Lim, {Yong Beom} and Taeil Kim and Lee, {Jae Wook} and Kim, {Seon Mi} and Kim, {Hee Joon} and Kimoon Kim and Park, {Jong Sang}",

year = "2002",

doi = "10.1021/bc025581r",

language = "English",

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journal = "Bioconjugate Chemistry",

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T1 - Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA

T2 - Noncovalent strategy in developing a gene delivery carrier

AU - Lim, Yong Beom

AU - Kim, Taeil

AU - Lee, Jae Wook

AU - Kim, Seon Mi

AU - Kim, Hee Joon

AU - Kim, Kimoon

AU - Park, Jong Sang

PY - 2002

Y1 - 2002

N2 - A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH2NH2+(CH2) 4NH3+]z], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.

AB - A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH2NH2+(CH2) 4NH3+]z], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.

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Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: Noncovalent strategy in developing a gene delivery carrier

Abstract

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