Selective position of individual cells without lysis on a circular window array using dielectrophoresis in a microfluidic device

Young Jin Jung, Taewoo Lee, Seungyeop Choi, Sei Young Lee, Jaehong Key, Yeong Min Yoo, Woo Jin Chang, Han Sung Kim, Sang Woo Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


We trapped individual cells between two circular windows using negative dielectrophoretic (DEP) force and then sequentially trapped them inside circular windows by positive DEP force without electrical lysis in a microfluidic device. Three parameters, (1) the transmembrane potential that determines the lysis of a cell, (2) individual cell size that affects the trapped position accuracy of the cell, and (3) the Clausius–Mossotti (CM) factor that decides the trapped efficiency of the cell, were characterized experimentally and numerically in this sequential cell trapping technique. In this characterization, we confirmed that the swap rate of applied voltage frequency, size similarity between the cell and circular window, and instantaneous change rate of Re(fCM) as a function of frequency were important factors in determining the selective position of individual cells without lysis. Our results provide useful suggestions for designing the structure of microfluidic DEP devices and optimizing variables required to manipulate individual cell trapping using both negative and positive DEP forces.

Original languageEnglish
Article number150
JournalMicrofluidics and Nanofluidics
Issue number9
Publication statusPublished - 2017 Sept 1

Bibliographical note

Funding Information:
Acknowledgements This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013R1A2A2A03005767, NRF-2017R1A2B2002076), Republic of Korea, and by the Yonsei University Future-leading Research Initiative (2016-22-0065, 2015-22-0070).

Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry


Dive into the research topics of 'Selective position of individual cells without lysis on a circular window array using dielectrophoresis in a microfluidic device'. Together they form a unique fingerprint.

Cite this