Selective aggregation mechanism of unmodified gold nanoparticles in detection of single nucleotide polymorphism

Kyungnam Cho, Yunhee Lee, Chang Ha Lee, Kangtaek Lee, Youngmin Kim, Hyoungwoo Choi, Pan Dong Ryu, So Yeong Lee, Sang Woo Joo

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


Detection of single point mutation based on the hybridization of oligonucleotides was performed using unmodified gold nanoparticles. The sequences of oligonucleotides were designed to detect the metastatic efficiency modifier signal-induced proliferation-associated gene 1 (Sipal). The detection step was monitored using UV-vis absorption spectroscopy, quasielastic light scattering, and zeta potential measurement. We observed that addition of DNAs into the suspension of unmodified gold nanoparticles could substantially aggregate the gold nanoparticles and change the color of solution. By changing the salt concentration in the presence of a phosphate buffer solution, we were able to selectively aggregate gold nanoparticles for the perfectly matched DNA, which enabled a detection of perfectly matched DNA from the single point-mutated one. Our results indicate that a change in the electrostatic interaction is responsible for the selective aggregation of gold nanoparticles upon the addition of DNA. This suggests a novel design principle for a rapid detection of the DNA sequence by controlling the electrostatic interactions between gold nanoparticles.

Original languageEnglish
Pages (from-to)8629-8633
Number of pages5
JournalJournal of Physical Chemistry C
Issue number23
Publication statusPublished - 2008 Jun 12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • General Energy
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films


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