Seed inocula for biohydrogen production from biodiesel solid residues

Gopalakrishnan Kumar, Chyi How Lay, Chen Yeon Chu, Jou Hsien Wu, Shih Chi Lee, Chiu Yue Lin

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21 Citations (Scopus)


This study aimed to optimize the hydrogen production from various seed sludges (two kinds of sewage sludges (S1, S2), cow dung (S3), granular sludge (S4) and effluent from condensed soluble molasses H 2 fermenter (S5)) and enhancement of hydrogen production via heat treatment for substrate and seed sludge by using the solid residues of biodiesel production (BDSR). Two batch assay tests were operated at a biodiesel solid residue concentration of 10 g/L, temperature of 55 °C and an initial cultivation pH of 8. The results showed that the peak hydrogen yield (HY) of 94.6 mL H 2/g volatile solid (VS) (4.1 mmolH 2/g VS) was obtained from S1 when substrate and seed sludge were both heat treated at 100 °C for 1 h. However, the peak hydrogen production rate (HPR) and specific hydrogen production rate (SHPR) of 1.48 L H 2/L-d and 0.30 L H 2/g VSS-d were obtained from S2 without any treatment. The heat treatment was found to increase the HY in both the cases of sewage sludges S1 and S2.The HY of 89.5 mL H 2/g VS (without treatment) was increased to 94.6 mL H 2/g VS and 82.6 mL H 2/g VS (without treatment) was increased to 85.7 mL H 2/g VS for S1 and S2. The soluble metabolic product (SMP) analysis showed that the fermentation followed mainly acetate-butyrate pathway with considerable production of ethanol. The total bioenergy production was calculated as 2.8 and 2.9 kJ/g VS for favorable hydrogen and ethanol production, respectively. The BDSR could be used as feedstock for dark fermentative hydrogen production.

Original languageEnglish
Pages (from-to)15489-15495
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number20
Publication statusPublished - 2012 Oct

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support by Taiwan’s Bureau of Energy (grant no. 99-D0204-3 ), Taiwan’s National Science Council ( NSC-99-2221-E-035-024-MY3 , NSC-99-2221-E-035-025-MY3 , NSC-99-2632-E-035-001-MY3 ), Feng Chia University ( FCU-10G27101 ), APEC Research Center for Advanced Biohydrogen Technology and the solid biodiesel processed waste support from Hua Neng Environmental Protection and Energy Technology Ltd, Taiwan.

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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