TY - JOUR
T1 - Role of Thalidomide on the Expression of OX40, 4-1BB, and GITR in T Cell Subsets
AU - Kim, B. S.
AU - Kim, J. Y.
AU - Kim, E. J.
AU - Lee, J. G.
AU - Joo, D. J.
AU - Huh, K. H.
AU - Kim, M. S.
AU - Kim, Y. S.
N1 - Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Background Thalidomide (TM) is known to have anti-cancer and anti-inflammatory properties; however, its mechanism on T cells is still unclear. We previously showed the immune modulatory effect of TM on T cells and its therapeutic effect on lupus nephritis models. Here we examined the changes in the expression of tumor necrosis factor receptor superfamilies (TNFRSFs), including OX40, 4-1BB, and glucocorticoid-induced TNFR-related protein (GITR) in T cell subsets by TM treatments. Methods Splenic naïve T cells (Tnaives) from C57BL/6 mice were sort-purified and cultured for CD4+ T cell proliferation and regulatory T cells (Tregs) conversion with TM treatments. All samples were analyzed by flow cytometry after stained with anti-mouse CD4, Foxp3, OX40, 4-1BB, or GITR antibodies. Results Expressions of OX40, 4-1BB, and GITR on CD4+ T cells showed a decreasing tendency by TM treatments. Especially, downregulation of these molecules on CD4+CFSElow T cells was significant in TM treatment groups. On the condition of Treg conversion, OX40 was downregulated significantly. In contrast, the expression of GITR was increased, and that of 4-1BB had shown no particular change under the condition of Treg. Conclusion Considering these results, TM may have an immune modulatory role through the T cell subset-specific change of OX40, 4-1BB, and GITR expression. Further study is required to elucidate the effect of thalidomide on T cells.
AB - Background Thalidomide (TM) is known to have anti-cancer and anti-inflammatory properties; however, its mechanism on T cells is still unclear. We previously showed the immune modulatory effect of TM on T cells and its therapeutic effect on lupus nephritis models. Here we examined the changes in the expression of tumor necrosis factor receptor superfamilies (TNFRSFs), including OX40, 4-1BB, and glucocorticoid-induced TNFR-related protein (GITR) in T cell subsets by TM treatments. Methods Splenic naïve T cells (Tnaives) from C57BL/6 mice were sort-purified and cultured for CD4+ T cell proliferation and regulatory T cells (Tregs) conversion with TM treatments. All samples were analyzed by flow cytometry after stained with anti-mouse CD4, Foxp3, OX40, 4-1BB, or GITR antibodies. Results Expressions of OX40, 4-1BB, and GITR on CD4+ T cells showed a decreasing tendency by TM treatments. Especially, downregulation of these molecules on CD4+CFSElow T cells was significant in TM treatment groups. On the condition of Treg conversion, OX40 was downregulated significantly. In contrast, the expression of GITR was increased, and that of 4-1BB had shown no particular change under the condition of Treg. Conclusion Considering these results, TM may have an immune modulatory role through the T cell subset-specific change of OX40, 4-1BB, and GITR expression. Further study is required to elucidate the effect of thalidomide on T cells.
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U2 - 10.1016/j.transproceed.2015.12.088
DO - 10.1016/j.transproceed.2015.12.088
M3 - Article
C2 - 27320601
AN - SCOPUS:84975144414
SN - 0041-1345
VL - 48
SP - 1270
EP - 1274
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 4
ER -
