RNA polymerase β-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp

K. S. Ko; T. Kuwahara; L. Haehwa; Y. J. Yoon; B. J. Kim; K. H. Lee; Y. Ohnishi; Y. H. Kook

doi:10.1111/j.1469-0691.2006.01553.x

RNA polymerase β-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp

K. S. Ko, T. Kuwahara, L. Haehwa, Y. J. Yoon, B. J. Kim, K. H. Lee, Y. Ohnishi, Y. H. Kook

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Partial rpoB sequences (317bp) of 11 species of Bacteroides, two Porphyromonas spp. and two Prevotella spp. were compared to delineate the genetic relationships among Bacteroides and closely related anaerobic species. The high level of inter-species sequence dissimilarities (7.6-20.8%) allowed the various Bacteroides spp. to be distinguished. The position of the Bacteroides distasonis and Bacteriodes merdae cluster in the rpoB tree was different from the position in the 16S rRNA gene tree. Based on rpoB sequence similarity and clustering in the rpoB tree, it was possible to correctly re-identify 80 clinical isolates of Bacteroides. In addition to two subgroups, cfiA -negative (division I) and cfiA-positive (division II), of Bacteroides fragilis isolates, two distinct subgroups were also found among Bacteroides ovatus and Bacteroides thetaiotaomicron isolates. Bacteroides genus-specific rpoB PCR and B. fragilis species-specific rpoB PCR allowed Bacteroides spp. to be differentiated from Porphyromonas and Prevotella spp., and also allowed B. fragilis to be differentiated from other non-fragilis Bacteroides spp. included in the present study.

Original language	English
Pages (from-to)	48-54
Number of pages	7
Journal	Clinical Microbiology and Infection
Volume	13
Issue number	1
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01553.x
Publication status	Published - 2007 Jan

Bibliographical note

Funding Information:
This work was supported by grant no. 03-2004-019-0 from the SNUH Research Fund and the BK21 Project for Medicine, Dentistry, and Pharmacy.

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1469-0691.2006.01553.x

Cite this

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title = "RNA polymerase β-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp",

abstract = "Partial rpoB sequences (317bp) of 11 species of Bacteroides, two Porphyromonas spp. and two Prevotella spp. were compared to delineate the genetic relationships among Bacteroides and closely related anaerobic species. The high level of inter-species sequence dissimilarities (7.6-20.8%) allowed the various Bacteroides spp. to be distinguished. The position of the Bacteroides distasonis and Bacteriodes merdae cluster in the rpoB tree was different from the position in the 16S rRNA gene tree. Based on rpoB sequence similarity and clustering in the rpoB tree, it was possible to correctly re-identify 80 clinical isolates of Bacteroides. In addition to two subgroups, cfiA -negative (division I) and cfiA-positive (division II), of Bacteroides fragilis isolates, two distinct subgroups were also found among Bacteroides ovatus and Bacteroides thetaiotaomicron isolates. Bacteroides genus-specific rpoB PCR and B. fragilis species-specific rpoB PCR allowed Bacteroides spp. to be differentiated from Porphyromonas and Prevotella spp., and also allowed B. fragilis to be differentiated from other non-fragilis Bacteroides spp. included in the present study.",

author = "Ko, {K. S.} and T. Kuwahara and L. Haehwa and Yoon, {Y. J.} and Kim, {B. J.} and Lee, {K. H.} and Y. Ohnishi and Kook, {Y. H.}",

note = "Funding Information: This work was supported by grant no. 03-2004-019-0 from the SNUH Research Fund and the BK21 Project for Medicine, Dentistry, and Pharmacy.",

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doi = "10.1111/j.1469-0691.2006.01553.x",

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AU - Ko, K. S.

AU - Kuwahara, T.

AU - Haehwa, L.

AU - Yoon, Y. J.

AU - Kim, B. J.

AU - Lee, K. H.

AU - Ohnishi, Y.

AU - Kook, Y. H.

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RNA polymerase β-subunit gene (rpoB) sequence analysis for the identification of Bacteroides spp

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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