A wound induces cell polarization, in which myosin II is localized at the rear end of individual cells in a migrating epithelial sheet of the Drosophila larval epidermis. Here, we use myosin localization to demonstrate that Rac1, Cdc42, and Rho1 are each required for cell polarization and directional sensing of the wound. The three GTPases are also required for actin cable formation at the wound leading edge. Rac1, Cdc42, and Rho1 act upstream of c-Jun N-terminal kinase (JNK) to organize actin assembly. These results highlight the similarities between the molecular mechanism of Drosophila wound healing and those of Drosophila embryonic dorsal closure and the chemotactic response of Dictyostelium and leukocytes.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2010 Apr 9|
Bibliographical noteFunding Information:
We thank M. Galko, D. Kiehart, and the Bloomington Drosophila Stock Center for fly stocks. We also thank members of the Choe lab for helpful discussions on the project. S.H.B. and Y.-C.K. were supported by Brain Korea 21, and Y.-C.K. was additionally supported by Seoul Science Fellowship. This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (7-2009-0297) to H.L. and Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean Government (MEST) ( R01-2007-000-11733-0 ) to K.-M.C.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology