Reversine induces multipotency of lineage-committed cells through epigenetic silencing of miR-133a

Munkyung Kim, Sang Ah Yi, Hyunwoo Lee, So Young Bang, Eun Kyung Park, Min Gyu Lee, Ki Hong Nam, Ji Hee Yoo, Dong Hoon Lee, Hyun Wook Ryu, So Hee Kwon, Jeung Whan Han

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14 Citations (Scopus)


Reversine has been shown to induce dedifferentiation of C2C12 murine myoblasts into multipotent progenitor cells. However, little is known about the key regulators mediating the dedifferentiation induced by reversine. Here, we show that large scale miRNA gene expression profiling of reversine-treated C2C12 myoblasts identifies a down-regulated miRNA, miR-133a, involved in dedifferentiation of myoblasts. Reversine treatment results in up- and down-regulated miRNA profiles. Among miRNAs affected by reversine, the level of muscle-specific miR-133a, which has been shown to be up-regulated during muscle development and to suppress differentiation into other lineages, is markedly reduced by treatment of C2C12 myoblasts with reversine. In parallel, reversine decreases the expression and recruitment of myogenic factor, SRF, to the enhancer regions of miR-133a. Sequentially, down-regulation of miR-133a by reversine is accompanied by a decrease in active histone modifications including trimethylation of histone H3K4 and H3K36, phosphorylation of H3S10, and acetylation of H3K14 on the miR-133a promoter, leading to dissociation of RNA polymerase II from the promoter. Furthermore, inhibition of miR-133a by transfection of C2C12 myoblasts with miR-133a inhibitor increases the expression of osteogenic lineage marker, Ogn, and adipotenic lineage marker, ApoE, similar to that in response to reversine. In contrast, the co-overexpression of miR-133a mimic reversed the effect of reversine on C2C12 myoblast dedifferentiation. Taken together, the results indicate that reversine induces a multipotency of C2C12 myoblasts by suppression of miR-133a expression through depletion of active histone modifications, and suggest that miR-133a is a potential miRNA regulating the reversine-induced dedifferentiation. Collectively, our findings provide a mechanistic rationale for the application of reversine to dedifferentiation of somatic cells.

Original languageEnglish
Pages (from-to)255-262
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 2014 Feb 28

Bibliographical note

Funding Information:
This research was supported by Medical Research Center programs ( 2012-0009849 ) and the Basic Science Research Program ( 2012013998 ) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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