Retinoic acid acts as a selective human IgA switch factor

Goo Young Seo; Young Saeng Jang; Jini Kim; Jongseon Choe; Hye Ju Han; Jeong Min Lee; Seong Ho Kang; Ki Jong Rhee; Seok Rae Park; Woan Sub Kim; Pyeung Hyeun Kim

doi:10.1016/j.humimm.2014.06.021

Retinoic acid acts as a selective human IgA switch factor

Goo Young Seo, Young Saeng Jang, Jini Kim, Jongseon Choe, Hye Ju Han, Jeong Min Lee, Seong Ho Kang, Ki Jong Rhee, Seok Rae Park, Woan Sub Kim, Pyeung Hyeun Kim

Biomedical Laboratory Science

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Retinoic acid (RA) is known to have several functions that lead to a potent mucosal IgA response. Nevertheless, its exact role in human IgA synthesis has yet to be elucidated. Thus, we investigated the role of RA in promoting IgA isotype switching in human B cells. We found that RA increased IgA production and the expression of germ-line IgA1 and IgA2 transcripts (GLTα1 and GLTα2). This induction occurred alongside an increase in the frequency of IgA1-secreting B cell clones, as assessed by limiting dilution analysis. Under the same conditions, RA did not increase IgM and IgG production. Am80, an agonist of RA receptor α (RARα), increased IgA production. In addition, RA activity was abrogated by LE540, an antagonist of RAR, suggesting that the RAR pathway is involved in RA-induced IgA production. Taken together, these results indicate that RA induces IgA isotype switching mainly through RARα in human B cells.

Original language	English
Pages (from-to)	923-929
Number of pages	7
Journal	Human Immunology
Volume	75
Issue number	8
DOIs	https://doi.org/10.1016/j.humimm.2014.06.021
Publication status	Published - 2014 Aug

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2013R1A1A2008536 to P.H.K. and No. 2011-0014492 to G.Y.S.). Studies were carried out in the Institute of Bioscience and Biotechnology at Kangwon National University.

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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title = "Retinoic acid acts as a selective human IgA switch factor",

abstract = "Retinoic acid (RA) is known to have several functions that lead to a potent mucosal IgA response. Nevertheless, its exact role in human IgA synthesis has yet to be elucidated. Thus, we investigated the role of RA in promoting IgA isotype switching in human B cells. We found that RA increased IgA production and the expression of germ-line IgA1 and IgA2 transcripts (GLTα1 and GLTα2). This induction occurred alongside an increase in the frequency of IgA1-secreting B cell clones, as assessed by limiting dilution analysis. Under the same conditions, RA did not increase IgM and IgG production. Am80, an agonist of RA receptor α (RARα), increased IgA production. In addition, RA activity was abrogated by LE540, an antagonist of RAR, suggesting that the RAR pathway is involved in RA-induced IgA production. Taken together, these results indicate that RA induces IgA isotype switching mainly through RARα in human B cells.",

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note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2013R1A1A2008536 to P.H.K. and No. 2011-0014492 to G.Y.S.). Studies were carried out in the Institute of Bioscience and Biotechnology at Kangwon National University. ",

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AU - Seo, Goo Young

AU - Jang, Young Saeng

AU - Kim, Jini

AU - Choe, Jongseon

AU - Han, Hye Ju

AU - Lee, Jeong Min

AU - Kang, Seong Ho

AU - Rhee, Ki Jong

AU - Park, Seok Rae

AU - Kim, Woan Sub

AU - Kim, Pyeung Hyeun

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2013R1A1A2008536 to P.H.K. and No. 2011-0014492 to G.Y.S.). Studies were carried out in the Institute of Bioscience and Biotechnology at Kangwon National University.

PY - 2014/8

Y1 - 2014/8

N2 - Retinoic acid (RA) is known to have several functions that lead to a potent mucosal IgA response. Nevertheless, its exact role in human IgA synthesis has yet to be elucidated. Thus, we investigated the role of RA in promoting IgA isotype switching in human B cells. We found that RA increased IgA production and the expression of germ-line IgA1 and IgA2 transcripts (GLTα1 and GLTα2). This induction occurred alongside an increase in the frequency of IgA1-secreting B cell clones, as assessed by limiting dilution analysis. Under the same conditions, RA did not increase IgM and IgG production. Am80, an agonist of RA receptor α (RARα), increased IgA production. In addition, RA activity was abrogated by LE540, an antagonist of RAR, suggesting that the RAR pathway is involved in RA-induced IgA production. Taken together, these results indicate that RA induces IgA isotype switching mainly through RARα in human B cells.

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Retinoic acid acts as a selective human IgA switch factor

Abstract

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