Remote ischemic conditioning by efuent collected from a novel isolated hindlimb model reduces infarct size in an isolated heart model

Young Jin Youn, Byung Su Yoo, Jung Woo Son, Jun Won Lee, Min Soo Ahn, Sung Gyun Ahn, Jang Young Kim, Seung Hwan Lee, Junghan Yoon, Young Woo Eom, Ji Eun Oh, Seong Kyung Choi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Background and Objectives: Experimental protocols for remote ischemic conditioning (RIC) utilize models in which a tourniquet is placed around the hindlimb or efuent is collected from an isolated heart. In analyzing the humoral factors that act as signal transducers in these models, sampled blood can be influenced by systemic responses, while the efuent from an isolated heart might differ from that of the hindlimb. Thus, we designed a new isolated hindlimb model for RIC and tested whether the efuent from this model could affect ischemia/reperfusion (IR) injury and if the reperfusion injury salvage kinase (RISK) and survivor activating factor enhancement (SAFE) pathways are involved in RIC. Materials and Methods: Afer positioning needles into the right iliac artery and vein of rats, Krebs-Henseleit buffer was perfused using a Langendorff apparatus, and efuent was collected. The RIC protocol consisted of 3 cycles of IR for 5 minutes. In the RIC efuent group, collected efuent was perfused in an isolated heart for 10 minutes before initiating IR injury. Results: Compared with the control group, the infarct area in the RIC efuent group was signifcantly smaller (31.2%±3.8% vs. 20.6%±1.8%, p<0.050), while phosphorylation of signal transducer and activation of transcription-3 (STAT-3) was signifcantly increased. However, there was a trend of increased phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 in this group. Conclusion: This is the frst study to investigate the effect of efuent from a new isolated hindlimb model afer RIC on IR injury in an isolated heart model. The RIC efuent was effective in reducing the IR injury, and the cardioprotective effect was associated with activation of the SAFE pathway.

Original languageEnglish
Pages (from-to)714-726
Number of pages13
JournalKorean Circulation Journal
Issue number5
Publication statusPublished - 2017 Sept

Bibliographical note

Funding Information:
This work was funded by the office of Research Affairs/University Industry Foundation (Grant No. 2014-51-0407).

Publisher Copyright:
© 2017. The Korean Society of Cardiology.

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine


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