Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Paz Merelo; Hathi Ram; Monica Pia Caggiano; Carolyn Ohno; Felix Ott; Daniel Straub; Moritz Graeff; Seok Keun Cho; Seong Wook Yang; Stephan Wenkel; Marcus G. Heisler

doi:10.1073/pnas.1516110113

Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Paz Merelo, Hathi Ram, Monica Pia Caggiano, Carolyn Ohno, Felix Ott, Daniel Straub, Moritz Graeff, Seok Keun Cho, Seong Wook Yang, Stephan Wenkel, Marcus G. Heisler

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

Original language	English
Pages (from-to)	11973-11978
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	113
Issue number	42
DOIs	https://doi.org/10.1073/pnas.1516110113
Publication status	Published - 2016 Oct 18

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© 2016, National Academy of Sciences. All rights reserved.

All Science Journal Classification (ASJC) codes

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10.1073/pnas.1516110113

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Merelo, P., Ram, H., Caggiano, M. P., Ohno, C., Ott, F., Straub, D., Graeff, M., Cho, S. K., Yang, S. W., Wenkel, S., & Heisler, M. G. (2016). Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity. Proceedings of the National Academy of Sciences of the United States of America, 113(42), 11973-11978. https://doi.org/10.1073/pnas.1516110113

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title = "Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity",

abstract = "A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.",

keywords = "Class II HD-ZIP, Class III HD-ZIP, Leaf morphogenesis, MIR165/166, Organ patterning",

author = "Paz Merelo and Hathi Ram and Caggiano, {Monica Pia} and Carolyn Ohno and Felix Ott and Daniel Straub and Moritz Graeff and Cho, {Seok Keun} and Yang, {Seong Wook} and Stephan Wenkel and Heisler, {Marcus G.}",

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Merelo, P, Ram, H, Caggiano, MP, Ohno, C, Ott, F, Straub, D, Graeff, M, Cho, SK, Yang, SW, Wenkel, S & Heisler, MG 2016, 'Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 42, pp. 11973-11978. https://doi.org/10.1073/pnas.1516110113

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AU - Merelo, Paz

AU - Ram, Hathi

AU - Caggiano, Monica Pia

AU - Ohno, Carolyn

AU - Ott, Felix

AU - Straub, Daniel

AU - Graeff, Moritz

AU - Cho, Seok Keun

AU - Yang, Seong Wook

AU - Wenkel, Stephan

AU - Heisler, Marcus G.

PY - 2016/10/18

Y1 - 2016/10/18

N2 - A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

AB - A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

KW - Class II HD-ZIP

KW - Class III HD-ZIP

KW - Leaf morphogenesis

KW - MIR165/166

KW - Organ patterning

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Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Abstract

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