Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17

Dockyu Kim, Young Soo Kim, Jae Woo Jung, Gerben J. Zylstra, Young Min Kim, Seong Ki Kim, Eungbin Kim

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13 Citations (Scopus)


Rhodococcus sp. strain DK17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. Although DK17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. The major accumulating compounds formed during the degradation of m- and p-xylene by DK17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass spectrometric and 1H nuclear magnetic resonance spectral techniques. Both xylene isomers were transformed to dihydroxylated compounds by what must be two successive hydroxylation events: m-xylene was converted to 2,4-dimethylresorcinol and p-xylene was converted to 2,5-dimethylhydroquinone. The rigorous structural identification of 2,4-dimethylresorcinol and 2,5-dimethylhydroquinone demonstrates that DK17 can perform distinct regioselective hydroxylations depending on the position of the substituent groups on the aromatic ring.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalFEMS Microbiology Letters
Issue number2
Publication statusPublished - 2003 Jun 27

Bibliographical note

Funding Information:
This work was supported by a grant from the Ministry of Science and Technology, Republic of Korea to E.K. (MG02-0301-005-1-0-0) through the 21C Frontier Microbial Genomics and Application Center Program. G.J.Z. wishes to thank NSF for their support through Grants MCB-0078465 and CHE-9810248.

All Science Journal Classification (ASJC) codes

  • General Medicine


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