Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation

Wern Joo Sohn; Hye In Jung; Min A. Choi; Jin Hyun Han; Gi Jeong Gwon; Hitoshi Yamamoto; Sanggyu Lee; Zae Young Ryoo; Eui Kyun Park; Hong In Shin; Han Sung Jung; Jae Young Kim

doi:10.1007/s00441-011-1204-8

Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation

Wern Joo Sohn, Hye In Jung, Min A. Choi, Jin Hyun Han, Gi Jeong Gwon, Hitoshi Yamamoto, Sanggyu Lee, Zae Young Ryoo, Eui Kyun Park, Hong In Shin, Han Sung Jung, Jae Young Kim

Department of Oral Biology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.

Original language	English
Pages (from-to)	265-273
Number of pages	9
Journal	Cell and Tissue Research
Volume	345
Issue number	2
DOIs	https://doi.org/10.1007/s00441-011-1204-8
Publication status	Published - 2011 Aug

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (R13-2008-009-01002-0)", and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2007-E00124). W.-J.Sohn.S.Lee.Z.Y.Ryoo School of Life Science and Biotechnology, Kyungpook National University, Daegu, South Korea

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology
Cell Biology

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title = "Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation",

abstract = "The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.",

author = "Sohn, {Wern Joo} and Jung, {Hye In} and Choi, {Min A.} and Han, {Jin Hyun} and Gwon, {Gi Jeong} and Hitoshi Yamamoto and Sanggyu Lee and Ryoo, {Zae Young} and Park, {Eui Kyun} and Shin, {Hong In} and Jung, {Han Sung} and Kim, {Jae Young}",

note = "Funding Information: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (R13-2008-009-01002-0){"}, and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2007-E00124). W.-J.Sohn.S.Lee.Z.Y.Ryoo School of Life Science and Biotechnology, Kyungpook National University, Daegu, South Korea",

year = "2011",

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doi = "10.1007/s00441-011-1204-8",

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AU - Jung, Hye In

AU - Choi, Min A.

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AU - Gwon, Gi Jeong

AU - Yamamoto, Hitoshi

AU - Lee, Sanggyu

AU - Ryoo, Zae Young

AU - Park, Eui Kyun

AU - Shin, Hong In

AU - Jung, Han Sung

AU - Kim, Jae Young

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PY - 2011/8

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Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation

Abstract

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