Recent advances in high-sensitivity detection methods for paper-based lateral-flow assay

Van Thuan Nguyen, Seungri Song, Seungkyung Park, Chulmin Joo

Research output: Contribution to journalReview articlepeer-review

170 Citations (Scopus)


Paper-based lateral-flow assays (LFAs) have achieved considerable commercial success and continue to have a significant impact on medical diagnostics and environmental monitoring. Conventional LFAs are typically performed by examining the color changes in the test bands by naked eye. However, for critical biochemical markers that are present in extremely small amounts in the clinical specimens, this readout method is not quantitative, and does not provide sufficient sensitivity or suitable detection limit for a reliable assay. Diverse technologies for high-sensitivity LFA detection have been developed and commercialization efforts are underway. In this review, we aim to provide a critical and objective overview of the recent progress in high-sensitivity LFA detection technologies, which involve the exploitation of the physical and chemical responses of transducing particles. The features and biomedical applications of the technologies, along with future prospects and challenges, are also discussed.

Original languageEnglish
Article number112015
JournalBiosensors and Bioelectronics
Publication statusPublished - 2020 Mar 15

Bibliographical note

Funding Information:
This work was supported by the research program of the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIT) (No. 2015R1A5A1037668 ).

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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