TY - JOUR
T1 - REBA HPV-ID® for efficient genotyping of human papillomavirus in clinical samples from Korean patients
AU - Kim, Sunghyun
AU - Lee, Dongsup
AU - Park, Sangjung
AU - Kim, Tae Ue
AU - Jeon, Bo Young
AU - Park, Kwang Hwa
AU - Lee, Hyeyoung
PY - 2012/8
Y1 - 2012/8
N2 - This study was conducted to evaluate the overall performance of a reverse blot hybridization-based assay, REBA HPV-ID® (Molecules and Diagnostics, Wonju, Korea) for genotyping human papillomaviruses (HPV). HPV Genotyping on 356 specimens examined cytologically was performed using the REBA HPV-ID®, and its results were compared with those obtained using the MyHPV DNA Chip® (Mygene, Seoul, Korea), DNA chip-based HPV genotyping assay. The results from this study showed that the positivity rate of the REBA HPV-ID® for abnormal cytological samples was higher (80.9%) than that of the MyHPV DNA chip (69.8%). In addition, the REBA HPV-ID® positivity rate with normal cytological samples was higher (64.4%) than that obtained using DNA chips (34.4%). Subsequently, sequence analysis was performed with specimens that generated conflicting test results. Sequence analysis confirmed that the specimens which were positive by REBA HPV-ID® did indeed contain HPV sequences. The results of this study suggest that the REBA HPV-ID® is a sensitive test for genotyping HPV of clinical specimens.
AB - This study was conducted to evaluate the overall performance of a reverse blot hybridization-based assay, REBA HPV-ID® (Molecules and Diagnostics, Wonju, Korea) for genotyping human papillomaviruses (HPV). HPV Genotyping on 356 specimens examined cytologically was performed using the REBA HPV-ID®, and its results were compared with those obtained using the MyHPV DNA Chip® (Mygene, Seoul, Korea), DNA chip-based HPV genotyping assay. The results from this study showed that the positivity rate of the REBA HPV-ID® for abnormal cytological samples was higher (80.9%) than that of the MyHPV DNA chip (69.8%). In addition, the REBA HPV-ID® positivity rate with normal cytological samples was higher (64.4%) than that obtained using DNA chips (34.4%). Subsequently, sequence analysis was performed with specimens that generated conflicting test results. Sequence analysis confirmed that the specimens which were positive by REBA HPV-ID® did indeed contain HPV sequences. The results of this study suggest that the REBA HPV-ID® is a sensitive test for genotyping HPV of clinical specimens.
UR - http://www.scopus.com/inward/record.url?scp=84862695185&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862695185&partnerID=8YFLogxK
U2 - 10.1002/jmv.23334
DO - 10.1002/jmv.23334
M3 - Article
C2 - 22711353
AN - SCOPUS:84862695185
SN - 0146-6615
VL - 84
SP - 1248
EP - 1253
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 8
ER -
