Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

Sun Mi Lee, Nalae Han, Rimi Lee, In Hong Choi, Yong Beom Park, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.

Original languageEnglish
Pages (from-to)56-61
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2016 Mar 15

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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