Rapid Bacterial Detection in Urine Using Laser Scattering and Deep Learning Analysis

Kwang Seob Lee, Hyung Jae Lim, Kyungnam Kim, Yeon Gyeong Park, Jae Woo Yoo, Dongeun Yong

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Images of laser scattering patterns generated by bacteria in urine are promising resources for deep learning. However, floating bacteria in urine produce dynamic scattering patterns and require deep learning of spatial and temporal features. We hypothesized that bacteria with variable bacterial densities and different Gram staining reactions would generate different speckle images. After deep learning of speckle patterns generated by various densities of bacteria in artificial urine, we validated the model in an independent set of clinical urine samples in a tertiary hospital. Even at a low bacterial density cutoff (1,000 CFU/mL), the model achieved a predictive accuracy of 90.9% for positive urine culture. At a cutoff of 50,000 CFU/mL, it showed a better accuracy of 98.5%. The model achieved satisfactory accuracy at both cutoff levels for predicting the Gram staining reaction. Considering only 30 min of analysis, our method appears as a new screening tool for predicting the presence of bacteria before urine culture.

Original languageEnglish
Article numbere01769-21
JournalMicrobiology spectrum
Issue number2
Publication statusPublished - 2022 Apr

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C1324 and HI21C0901); by the BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (H-GUARD_2018M3A6B2057322).

Publisher Copyright:
© 2022 Lee et al.

All Science Journal Classification (ASJC) codes

  • Physiology
  • Ecology
  • Immunology and Microbiology(all)
  • Genetics
  • Microbiology (medical)
  • Cell Biology
  • Infectious Diseases


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