Rapid and sensitive detection of bacteria pathogen based on microfluidic enrichement with label-free nanobiosensor

A highly sensitive nanobiosensor combining a Concanavalina A (Con A) functionalized microfluidic chip is reported for rapid detection of bacteria pathogen based on isothermal amplification. The microfluidic chip including asymmetric herringbone structures was employed to enrich microorganisms of interests and extract DNAs. An optical nanobiosensor-based isothermal nucleic acid amplification technique was used to carry out the simultaneous amplification and detection of target nucleic acid in a label-free and real-time manner. Salmonella enterica serotype Typhimurium can be detected at the concentration as low as 10 colony-forming units (CFU) in 1ml PBS buffer and 50 CFU in 10 ml urine sample. The developed nanobiosensing platform has potential to provide a low-cost and sensitive tool for rapid bacterial detection in various samples.