R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo

Nam Hee Kim; Ngoc Bich Pham; Ronald J. Quinn; Ho Jeong Kwon

doi:10.1016/j.bbrc.2010.07.025

R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo

Nam Hee Kim, Ngoc Bich Pham, Ronald J. Quinn, Ho Jeong Kwon

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

R-(-)-β-O-methylsynephrine (OMe-Syn) is an active compound isolated from a plant of the Rutaceae family. We conducted cell proliferation assays on various cell lines and found that OMe-Syn more strongly inhibited the growth of human umbilical vein endothelial cells (HUVECs) than that of other normal and cancer cell lines tested. In angiogenesis assays, it inhibited vascular endothelial growth factor (VEGF)-induced invasion and tube formation of HUVECs with no toxicity. The anti-angiogenic activity of OMe-Syn was also validated in vivo using the chorioallantonic membrane (CAM) assay in growing chick embryos. Expression of the growth factors VEGF, hepatocyte growth factor, and basic fibroblast growth factor was suppressed by OMe-Syn in a dose-dependent manner. Taken together, our results indicate that this compound could be a novel basis for a small molecule targeting angiogenesis.

Original language	English
Pages (from-to)	20-23
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	399
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2010.07.025
Publication status	Published - 2010 Aug

Bibliographical note

Funding Information:
This study was partly supported by grants from the Translational Research Center for Protein Function Control , the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) ( 2009-0083522, 2009-0092964 , 2010-0017984 ), the National R&D Program for Cancer Control, Ministry of Health & Welfare ( 0620350 ) ,and from the Brain Korea 21 project, Republic of Korea.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo",

abstract = "R-(-)-β-O-methylsynephrine (OMe-Syn) is an active compound isolated from a plant of the Rutaceae family. We conducted cell proliferation assays on various cell lines and found that OMe-Syn more strongly inhibited the growth of human umbilical vein endothelial cells (HUVECs) than that of other normal and cancer cell lines tested. In angiogenesis assays, it inhibited vascular endothelial growth factor (VEGF)-induced invasion and tube formation of HUVECs with no toxicity. The anti-angiogenic activity of OMe-Syn was also validated in vivo using the chorioallantonic membrane (CAM) assay in growing chick embryos. Expression of the growth factors VEGF, hepatocyte growth factor, and basic fibroblast growth factor was suppressed by OMe-Syn in a dose-dependent manner. Taken together, our results indicate that this compound could be a novel basis for a small molecule targeting angiogenesis.",

author = "Kim, {Nam Hee} and Pham, {Ngoc Bich} and Quinn, {Ronald J.} and Kwon, {Ho Jeong}",

note = "Funding Information: This study was partly supported by grants from the Translational Research Center for Protein Function Control , the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) ( 2009-0083522, 2009-0092964 , 2010-0017984 ), the National R&D Program for Cancer Control, Ministry of Health & Welfare ( 0620350 ) ,and from the Brain Korea 21 project, Republic of Korea.",

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AU - Quinn, Ronald J.

AU - Kwon, Ho Jeong

N1 - Funding Information: This study was partly supported by grants from the Translational Research Center for Protein Function Control , the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) ( 2009-0083522, 2009-0092964 , 2010-0017984 ), the National R&D Program for Cancer Control, Ministry of Health & Welfare ( 0620350 ) ,and from the Brain Korea 21 project, Republic of Korea.

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R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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