TY - JOUR
T1 - Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling
AU - Jadaliha, Mahdieh
AU - Lee, Hyoung Joo
AU - Pakzad, Mohammad
AU - Fathi, Ali
AU - Jeong, Seul Ki
AU - Cho, Sang Yun
AU - Baharvand, Hossein
AU - Paik, Young Ki
AU - Salekdeh, Ghasem Hosseini
PY - 2012/6/18
Y1 - 2012/6/18
N2 - Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation.
AB - Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation.
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U2 - 10.1371/journal.pone.0038532
DO - 10.1371/journal.pone.0038532
M3 - Article
C2 - 22723866
AN - SCOPUS:84862497291
SN - 1932-6203
VL - 7
JO - PLoS One
JF - PLoS One
IS - 6
M1 - e38532
ER -