TY - JOUR
T1 - Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
AU - Antkowiak, Maciej
AU - Torres-Mapa, Maria Leilani
AU - Dholakia, Kishan
AU - Gunn-Moore, Frank J.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles.
AB - We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles.
UR - http://www.scopus.com/inward/record.url?scp=79960509097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960509097&partnerID=8YFLogxK
U2 - 10.1364/BOE.1.000414
DO - 10.1364/BOE.1.000414
M3 - Article
AN - SCOPUS:79960509097
SN - 2156-7085
VL - 1
SP - 414
EP - 424
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 2
ER -