Quantification of bacteria in mouth-rinsing solution for the diagnosis of periodontal disease

Jeong Hwa Kim, Jae Woon Oh, Young Lee, Jeong Ho Yun, Seong Ho Choi, Dong Woon Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


This study aimed to evaluate the feasibility of diagnosing periodontitis via the identification of 18 bacterial species in mouth-rinse samples. Patients (n = 110) who underwent dental exam-inations in the Department of Periodontology at the Veterans Health Service Medical Center between 2018 and 2019 were included. They were divided into healthy and periodontitis groups. The overall number of bacteria, and those of 18 specific bacteria, were determined via real-time poly-merase chain reaction in 92 mouth-rinse samples. Differences between groups were evaluated through logistic regression after adjusting for sex, age, and smoking history. There was a significant difference in the prevalence (healthy vs. periodontitis group) of Aggregatibacter actinomycetemcomi-tans (2.9% vs. 13.5%), Treponema denticola (42.9% vs. 69.2%), and Prevotella nigrescens (80% vs. 2.7%). Levels of Treponema denticola, Prevotella nigrescens, and Streptococcus mitis were significantly associated with severe periodontitis. We demonstrated the feasibility of detecting periopathogenic bacteria in mouth-rinse samples obtained from patients with periodontitis. As we did not comprehen-sively assess all periopathogenic bacteria, further studies are required to assess the potential of oral-rinsing solutions to indicate oral infection risk and the need to improve oral hygiene, and to serve as a complementary method for periodontal disease diagnosis.

Original languageEnglish
Article number891
Pages (from-to)1-18
Number of pages18
JournalJournal of Clinical Medicine
Issue number4
Publication statusPublished - 2021 Feb 2

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • General Medicine


Dive into the research topics of 'Quantification of bacteria in mouth-rinsing solution for the diagnosis of periodontal disease'. Together they form a unique fingerprint.

Cite this