Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling

Francesca Puppo; Virginie Thomé; Anne Catherine Lhoumeau; Marie Cibois; Akanksha Gangar; Frédérique Lembo; Edwige Belotti; Sylvie Marchetto; Patrick Lécine; Thomas Prébet; Michael Sebbagh; Won Sik Shin; Seung Taek Lee; Laurent Kodjabachian; Jean Paul Borg

doi:10.1038/embor.2010.185

Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling

Francesca Puppo, Virginie Thomé, Anne Catherine Lhoumeau, Marie Cibois, Akanksha Gangar, Frédérique Lembo, Edwige Belotti, Sylvie Marchetto, Patrick Lécine, Thomas Prébet, Michael Sebbagh, Won Sik Shin, Seung Taek Lee, Laurent Kodjabachian, Jean Paul Borg

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakenedβ-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require Î 2-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	EMBO Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/embor.2010.185
Publication status	Published - 2011 Jan

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics

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10.1038/embor.2010.185

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title = "Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling",

abstract = "The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakenedβ-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require {\^I} 2-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.",

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doi = "10.1038/embor.2010.185",

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AU - Puppo, Francesca

AU - Thomé, Virginie

AU - Lhoumeau, Anne Catherine

AU - Cibois, Marie

AU - Gangar, Akanksha

AU - Lembo, Frédérique

AU - Belotti, Edwige

AU - Marchetto, Sylvie

AU - Lécine, Patrick

AU - Prébet, Thomas

AU - Sebbagh, Michael

AU - Shin, Won Sik

AU - Lee, Seung Taek

AU - Kodjabachian, Laurent

AU - Borg, Jean Paul

PY - 2011/1

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AB - The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakenedβ-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require Î 2-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.

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Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling

Abstract

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