Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice

Soonjae Hwang; Minjeong Jo; Ju Eun Hong; Chan Oh Park; Chang Gun Lee; Ki Jong Rhee

doi:10.3390/ijms21030857

Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice

Soonjae Hwang, Minjeong Jo, Ju Eun Hong, Chan Oh Park, Chang Gun Lee, Ki Jong Rhee

Biomedical Laboratory Science

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Chronic inflammation has been linked to colitis-associated colorectal cancer in humans. The human symbiont enterotoxigenic Bacteroides fragilis (ETBF), a pro-carcinogenic bacterium, has the potential to initiate and/or promote colorectal cancer. Antibiotic treatment of ETBF has shown promise in decreasing colonic polyp formation in murine models of colon cancer. However, there are no reported natural products that have shown efficacy in decreasing polyp burden. In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis. Zerumbone significantly reduced the severity of disease activity index (DAI) scores as well as several parameters of colonic inflammation (i.e., colon weight, colon length, cecum weight and spleen weight). In addition, inflammation of the colon and cecum as well as hyperplasia was reduced. Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.

Original language	English
Article number	857
Journal	International journal of molecular sciences
Volume	21
Issue number	3
DOIs	https://doi.org/10.3390/ijms21030857
Publication status	Published - 2020 Feb 1

Bibliographical note

Funding Information:
Funding: This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF‐2017‐Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future‐Leading Research Initiative of 2017 (2017‐52‐ 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH).

Funding Information:
This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF-2017-Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52- 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/ijms21030857

Cite this

@article{0efc66dc31c74298b87d4f122faceaf0,

title = "Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice",

abstract = "Chronic inflammation has been linked to colitis-associated colorectal cancer in humans. The human symbiont enterotoxigenic Bacteroides fragilis (ETBF), a pro-carcinogenic bacterium, has the potential to initiate and/or promote colorectal cancer. Antibiotic treatment of ETBF has shown promise in decreasing colonic polyp formation in murine models of colon cancer. However, there are no reported natural products that have shown efficacy in decreasing polyp burden. In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis. Zerumbone significantly reduced the severity of disease activity index (DAI) scores as well as several parameters of colonic inflammation (i.e., colon weight, colon length, cecum weight and spleen weight). In addition, inflammation of the colon and cecum as well as hyperplasia was reduced. Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.",

author = "Soonjae Hwang and Minjeong Jo and Hong, {Ju Eun} and Park, {Chan Oh} and Lee, {Chang Gun} and Rhee, {Ki Jong}",

note = "Funding Information: Funding: This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF‐2017‐Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future‐Leading Research Initiative of 2017 (2017‐52‐ 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH). Funding Information: This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF-2017-Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52- 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = feb,

day = "1",

doi = "10.3390/ijms21030857",

language = "English",

volume = "21",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "3",

}

TY - JOUR

T1 - Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice

AU - Hwang, Soonjae

AU - Jo, Minjeong

AU - Hong, Ju Eun

AU - Park, Chan Oh

AU - Lee, Chang Gun

AU - Rhee, Ki Jong

N1 - Funding Information: Funding: This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF‐2017‐Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future‐Leading Research Initiative of 2017 (2017‐52‐ 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH). Funding Information: This research was funded by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 to KJR) and NRF-2017-Fostering Core Leaders of the Future Basic Science Program/Global Ph.D. Fellowship Program (2017H1A2A1045727 to MJ). In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52- 0078 to KJR). In addition, supported in part by the Yonsei University Research Fund of 2019 (to SH). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/2/1

Y1 - 2020/2/1

N2 - Chronic inflammation has been linked to colitis-associated colorectal cancer in humans. The human symbiont enterotoxigenic Bacteroides fragilis (ETBF), a pro-carcinogenic bacterium, has the potential to initiate and/or promote colorectal cancer. Antibiotic treatment of ETBF has shown promise in decreasing colonic polyp formation in murine models of colon cancer. However, there are no reported natural products that have shown efficacy in decreasing polyp burden. In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis. Zerumbone significantly reduced the severity of disease activity index (DAI) scores as well as several parameters of colonic inflammation (i.e., colon weight, colon length, cecum weight and spleen weight). In addition, inflammation of the colon and cecum as well as hyperplasia was reduced. Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.

AB - Chronic inflammation has been linked to colitis-associated colorectal cancer in humans. The human symbiont enterotoxigenic Bacteroides fragilis (ETBF), a pro-carcinogenic bacterium, has the potential to initiate and/or promote colorectal cancer. Antibiotic treatment of ETBF has shown promise in decreasing colonic polyp formation in murine models of colon cancer. However, there are no reported natural products that have shown efficacy in decreasing polyp burden. In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis. Zerumbone significantly reduced the severity of disease activity index (DAI) scores as well as several parameters of colonic inflammation (i.e., colon weight, colon length, cecum weight and spleen weight). In addition, inflammation of the colon and cecum as well as hyperplasia was reduced. Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.

UR - http://www.scopus.com/inward/record.url?scp=85078894726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85078894726&partnerID=8YFLogxK

U2 - 10.3390/ijms21030857

DO - 10.3390/ijms21030857

M3 - Article

C2 - 32013191

AN - SCOPUS:85078894726

SN - 1661-6596

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 3

M1 - 857

ER -

Protective effects of zerumbone on colonic tumorigenesis in enterotoxigenic bacteroides fragilis (ETBF)-colonized AOM/DSS BALB/c mice

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this