Prosthecobacter algae sp. nov., isolated from activated sludge using algal metabolites

A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04^T, was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04^T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is closely related to Prosthecobacter dejongeii DSM 12251^T (98.6% sequence similarity), Prosthecobacter fusiformis ATCC 25309T (97.9 %), Prosthecobacter debontii DSM 14044^T (97.5 %), Prosthecobacter vanneervenii DSM 12252^T (94.7 %) and Prosthecobacter fluviatilis KCTC 22182^T (93.7 %). The G+C content of the genomic DNA of strain EBTL04^T was 62.7 mol%. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04^T contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with C_{16: 1}ω5c, iso-C_{14: 0}, C_{16: 0}, anteiso- C_{15: 0} and C_{14: 0} as the major components supported the classification of strain EBTL04^T in the genus Prosthecobacter. Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04^T was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04^T should be considered to represent a novel species of the genus Prosthecobacter, for which the name Prosthecobacter algae sp. nov. is proposed. The type strain is EBTL04^T (5KCTC 23681^T5JCM 18053^T).