Proof-of-concept study of vertical augmentation using block-type allogenic bone grafts: A preclinical experimental study on rabbit calvaria

Ji Sun Lee, Jung Seok Lee, Myung Hun Kang, Ui Won Jung, Seong Ho Choi, Kyoo Sung Cho

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7 Citations (Scopus)


This study aimed to quantify the healing following vertical augmentation of allogenic bone blocks with/without recombinant human bone morphogenetic protein-2 (rhBMP-2) on rabbit calvaria. Experiments were performed using allogenic bone blocks which were grafted bilaterally with or without rhBMP-2 on 20 rabbit calvaria, and these animals were divided to four groups according to the use of rhBMP-2 and healing periods (2 and 8 weeks; n = 10 in each group). Onlay-type bone blocks (8 mm in diameter and 5 mm high) were fixed with a self-tapping screw after removing the cortex in the control group, and the same protocol was applied with the addition of soaking the bone blocks with rhBMP-2 for 15 min in the test group. Radiographic and histologic analyses were performed after 2 or 8 weeks to evaluate the volumetric stability and bone regeneration within the grafted area. The radiographic analysis revealed that the height of the allogenic bone block decreased but its volume was maintained from 2 to 8 weeks in both the control and test groups. The histologic results demonstrated a statistically significant increase in new bone area in the test group, especially in the lower region adjacent to the preexisting calvarial floor. The amount of newly formed bone in all regions of the augmented bone blocks in both the control and test groups was greater at 8 weeks than at 2 weeks. In conclusion, the vertically grafted allogenic bone block maintained its volume with new bone formation, and this was accelerated by the addition of rhBMP-2. These findings indicate that allogenic bone block soaked with rhBMP-2 could be a useful candidate biomaterial for vertical augmentation.

Original languageEnglish
Pages (from-to)2700-2707
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number7
Publication statusPublished - 2018 Oct

Bibliographical note

Funding Information:
Correspondence to: J.-S. Lee; Contract grant sponsor: National Research Foundation of Korea (NRF), Ministry of Education (Basic Science Research Program); contract grant number: 2016R1C1B2015427

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering


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