Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong; Dong Woo Lee; Sang Jae Lee; Eun Ah Choe; Seong Bo Kim; Yoon Hee Lee; Chan Ick Cheigh; Yu Ryang Pyun

doi:10.1007/s10529-006-9277-2

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong, Dong Woo Lee, Sang Jae Lee, Eun Ah Choe, Seong Bo Kim, Yoon Hee Lee, Chan Ick Cheigh, Yu Ryang Pyun

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27 Citations (Scopus)

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t_1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original language	English
Pages (from-to)	569-574
Number of pages	6
Journal	Biotechnology Letters
Volume	29
Issue number	4
DOIs	https://doi.org/10.1007/s10529-006-9277-2
Publication status	Published - 2007 Apr

Bibliographical note

Funding Information:
Acknowledgements This work was supported by grant 2001-2-0109 from the Korea Science and Engineering Foundation and grant AIC-08-02 from the Ministry of Commerce, Industry and Energy, Korea.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana",

abstract = "Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.",

author = "Hong, {Young Ho} and Lee, {Dong Woo} and Lee, {Sang Jae} and Choe, {Eun Ah} and Kim, {Seong Bo} and Lee, {Yoon Hee} and Cheigh, {Chan Ick} and Pyun, {Yu Ryang}",

note = "Funding Information: Acknowledgements This work was supported by grant 2001-2-0109 from the Korea Science and Engineering Foundation and grant AIC-08-02 from the Ministry of Commerce, Industry and Energy, Korea.",

year = "2007",

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doi = "10.1007/s10529-006-9277-2",

language = "English",

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T1 - Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

AU - Hong, Young Ho

AU - Lee, Dong Woo

AU - Lee, Sang Jae

AU - Choe, Eun Ah

AU - Kim, Seong Bo

AU - Lee, Yoon Hee

AU - Cheigh, Chan Ick

AU - Pyun, Yu Ryang

N1 - Funding Information: Acknowledgements This work was supported by grant 2001-2-0109 from the Korea Science and Engineering Foundation and grant AIC-08-02 from the Ministry of Commerce, Industry and Energy, Korea.

PY - 2007/4

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N2 - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

AB - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

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Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Abstract

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