Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels

Eun Jae Chung; Ji Suk Choi; Jisoo Shin; Ha Na Cho; Suyeon Kim; Ji Yong Park; Yun sang Lee; Yong il Kim; Hong Gyun Wu; Seung Woo Cho; Seong Keun Kwon

doi:10.1016/j.jiec.2020.06.014

Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels

Eun Jae Chung, Ji Suk Choi, Jisoo Shin, Ha Na Cho, Suyeon Kim, Ji Yong Park, Yun sang Lee, Yong il Kim, Hong Gyun Wu, Seung Woo Cho, Seong Keun Kwon

Research output: Contribution to journal › Article › peer-review

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Abstract

Most patients with head and neck cancer experience salivary gland dysfunction after chemo- and radiotherapies. There is currently no available treatment for this condition. Intervention with stem cell therapy has emerged as a promising approach. Although some progress has been made with systemic delivery of stem cells, it remains challenging to deliver a sufficient number of stem cells to the damaged tissue. Furthermore, local delivery of stem cells involves frequent cell loss. Herein, we evaluated the ability of adipose-derived stem cells (ASCs) with or without hydrogel to prevent salivary gland damage. Salivary gland cells were isolated from irradiated mouse submandibular glands. These cells exhibited higher expression levels of amylase, mucin, and aquaporin-5 when co-cultured with ASCs. Local delivery of ASCs into the salivary gland, using in situ-forming hyaluronic acid-based hydrogel (HA gel) as a carrier, revealed that ASCs remained at the injection site for a longer duration, compared with ASCs that were injected without HA gel. The salivary gland exhibited better function and morphology when ASCs were injected using an HA gel. In conclusion, retention of locally delivered ASCs by HA gels can enhance the paracrine effect of ASCs, thereby preventing irradiation-induced damage to the salivary gland and subsequent dysfunction.

Original language	English
Pages (from-to)	47-57
Number of pages	11
Journal	Journal of Industrial and Engineering Chemistry
Volume	90
DOIs	https://doi.org/10.1016/j.jiec.2020.06.014
Publication status	Published - 2020 Oct 25

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2017R1A2B4011229, SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2019M3A9H1103617, SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277, SK Kwon). The authors wish to thank Jae-Yol Lim (Yonsei University College of Medicine, Seoul, Republic of Korea) and Jeong-Seok Choi (Inha University, College of Medicine, Incheon, Republic of Korea) for techniques of animal experiment.

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea ( NRF ) funded by the Korea government (MSIP) ( NRF-2017R1A2B4011229 , SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government ( MSIT ) (No. 2019M3A9H1103617 , SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277 , SK Kwon).

Publisher Copyright:
© 2020 The Korean Society of Industrial and Engineering Chemistry

All Science Journal Classification (ASJC) codes

Chemical Engineering(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jiec.2020.06.014

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Chung, E. J., Choi, J. S., Shin, J., Cho, H. N., Kim, S., Park, J. Y., Lee, Y. S., Kim, Y. I., Wu, H. G., Cho, S. W., & Kwon, S. K. (2020). Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels. Journal of Industrial and Engineering Chemistry, 90, 47-57. https://doi.org/10.1016/j.jiec.2020.06.014

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title = "Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels",

abstract = "Most patients with head and neck cancer experience salivary gland dysfunction after chemo- and radiotherapies. There is currently no available treatment for this condition. Intervention with stem cell therapy has emerged as a promising approach. Although some progress has been made with systemic delivery of stem cells, it remains challenging to deliver a sufficient number of stem cells to the damaged tissue. Furthermore, local delivery of stem cells involves frequent cell loss. Herein, we evaluated the ability of adipose-derived stem cells (ASCs) with or without hydrogel to prevent salivary gland damage. Salivary gland cells were isolated from irradiated mouse submandibular glands. These cells exhibited higher expression levels of amylase, mucin, and aquaporin-5 when co-cultured with ASCs. Local delivery of ASCs into the salivary gland, using in situ-forming hyaluronic acid-based hydrogel (HA gel) as a carrier, revealed that ASCs remained at the injection site for a longer duration, compared with ASCs that were injected without HA gel. The salivary gland exhibited better function and morphology when ASCs were injected using an HA gel. In conclusion, retention of locally delivered ASCs by HA gels can enhance the paracrine effect of ASCs, thereby preventing irradiation-induced damage to the salivary gland and subsequent dysfunction.",

author = "Chung, {Eun Jae} and Choi, {Ji Suk} and Jisoo Shin and Cho, {Ha Na} and Suyeon Kim and Park, {Ji Yong} and Lee, {Yun sang} and Kim, {Yong il} and Wu, {Hong Gyun} and Cho, {Seung Woo} and Kwon, {Seong Keun}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2017R1A2B4011229, SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2019M3A9H1103617, SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277, SK Kwon). The authors wish to thank Jae-Yol Lim (Yonsei University College of Medicine, Seoul, Republic of Korea) and Jeong-Seok Choi (Inha University, College of Medicine, Incheon, Republic of Korea) for techniques of animal experiment. Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea ( NRF ) funded by the Korea government (MSIP) ( NRF-2017R1A2B4011229 , SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government ( MSIT ) (No. 2019M3A9H1103617 , SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277 , SK Kwon). Publisher Copyright: {\textcopyright} 2020 The Korean Society of Industrial and Engineering Chemistry",

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doi = "10.1016/j.jiec.2020.06.014",

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T1 - Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels

AU - Chung, Eun Jae

AU - Choi, Ji Suk

AU - Shin, Jisoo

AU - Cho, Ha Na

AU - Kim, Suyeon

AU - Park, Ji Yong

AU - Lee, Yun sang

AU - Kim, Yong il

AU - Wu, Hong Gyun

AU - Cho, Seung Woo

AU - Kwon, Seong Keun

N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (NRF-2017R1A2B4011229, SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2019M3A9H1103617, SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277, SK Kwon). The authors wish to thank Jae-Yol Lim (Yonsei University College of Medicine, Seoul, Republic of Korea) and Jeong-Seok Choi (Inha University, College of Medicine, Incheon, Republic of Korea) for techniques of animal experiment. Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea ( NRF ) funded by the Korea government (MSIP) ( NRF-2017R1A2B4011229 , SK Kwon and 2018M3A9H1021382, SW Cho). Also, this research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government ( MSIT ) (No. 2019M3A9H1103617 , SK Kwon) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277 , SK Kwon). Publisher Copyright: © 2020 The Korean Society of Industrial and Engineering Chemistry

PY - 2020/10/25

Y1 - 2020/10/25

N2 - Most patients with head and neck cancer experience salivary gland dysfunction after chemo- and radiotherapies. There is currently no available treatment for this condition. Intervention with stem cell therapy has emerged as a promising approach. Although some progress has been made with systemic delivery of stem cells, it remains challenging to deliver a sufficient number of stem cells to the damaged tissue. Furthermore, local delivery of stem cells involves frequent cell loss. Herein, we evaluated the ability of adipose-derived stem cells (ASCs) with or without hydrogel to prevent salivary gland damage. Salivary gland cells were isolated from irradiated mouse submandibular glands. These cells exhibited higher expression levels of amylase, mucin, and aquaporin-5 when co-cultured with ASCs. Local delivery of ASCs into the salivary gland, using in situ-forming hyaluronic acid-based hydrogel (HA gel) as a carrier, revealed that ASCs remained at the injection site for a longer duration, compared with ASCs that were injected without HA gel. The salivary gland exhibited better function and morphology when ASCs were injected using an HA gel. In conclusion, retention of locally delivered ASCs by HA gels can enhance the paracrine effect of ASCs, thereby preventing irradiation-induced damage to the salivary gland and subsequent dysfunction.

AB - Most patients with head and neck cancer experience salivary gland dysfunction after chemo- and radiotherapies. There is currently no available treatment for this condition. Intervention with stem cell therapy has emerged as a promising approach. Although some progress has been made with systemic delivery of stem cells, it remains challenging to deliver a sufficient number of stem cells to the damaged tissue. Furthermore, local delivery of stem cells involves frequent cell loss. Herein, we evaluated the ability of adipose-derived stem cells (ASCs) with or without hydrogel to prevent salivary gland damage. Salivary gland cells were isolated from irradiated mouse submandibular glands. These cells exhibited higher expression levels of amylase, mucin, and aquaporin-5 when co-cultured with ASCs. Local delivery of ASCs into the salivary gland, using in situ-forming hyaluronic acid-based hydrogel (HA gel) as a carrier, revealed that ASCs remained at the injection site for a longer duration, compared with ASCs that were injected without HA gel. The salivary gland exhibited better function and morphology when ASCs were injected using an HA gel. In conclusion, retention of locally delivered ASCs by HA gels can enhance the paracrine effect of ASCs, thereby preventing irradiation-induced damage to the salivary gland and subsequent dysfunction.

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Prevention of irradiation-induced damage to salivary glands by local delivery of adipose-derived stem cells via hyaluronic acid-based hydrogels

Abstract

Bibliographical note

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UN SDGs

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