TY - JOUR
T1 - Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea
AU - Choi, Young Hwa
AU - Ngamskulrungroj, Popchai
AU - Varma, Ashok
AU - Sionov, Edward
AU - Hwang, Soo Myung
AU - Carriconde, Fabian
AU - Meyer, Wieland
AU - Litvintseva, Anastasia P.
AU - Lee, Wee Gyo
AU - Shin, Jong Hee
AU - Kim, Eui Chong
AU - Lee, Kyung Won
AU - Choi, Tae Yeal
AU - Lee, Yeong Seon
AU - Kwon-Chung, Kyung J.
PY - 2010/9
Y1 - 2010/9
N2 - PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96% were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4% were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6%) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93% of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8%) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20%) (P=0.009). Unlike the Chinese strains, a majority (60%) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.
AB - PCR fingerprinting and multilocus sequence typing were applied to determine the major molecular types of the Cryptococcus neoformansCryptococcus gattii species complex in the Republic of Korea. Of the 78 strains isolated from patients diagnosed with cryptococcosis between 1990 and 2008, 96% were C. neoformans serotype A, mating type MATα and molecular type VNI. The remaining 4% were C. gattii, serotype B, mating type MATα and either molecular type VGIIb or VGIII. Of the 62 strains with known HIV status, only 14 (22.6%) were isolated from HIV-positive patients and belonged to molecular type VNI. Remarkably, 93% of the C. neoformans isolates had identical PCR fingerprint profiles with the VNIc genotype that has been identified recently as the major genotype among C. neoformans strains in China. Most strains (81.8%) of the VNIc genotype were associated with non-HIV patients compared with strains of the non-VNIc genotype (20%) (P=0.009). Unlike the Chinese strains, a majority (60%) of the non-HIV patients infected with strains of the VNIc genotype in the Republic of Korea had serious underlying conditions, with cancer and liver disease being the most common. This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis.
UR - http://www.scopus.com/inward/record.url?scp=77955802922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955802922&partnerID=8YFLogxK
U2 - 10.1111/j.1567-1364.2010.00648.x
DO - 10.1111/j.1567-1364.2010.00648.x
M3 - Article
C2 - 20561059
AN - SCOPUS:77955802922
SN - 1567-1356
VL - 10
SP - 769
EP - 778
JO - FEMS Yeast Research
JF - FEMS Yeast Research
IS - 6
ER -
