Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital

Hee Jeong Yun; Sang Won Lee; Gyu Man Yoon; Su Yeon Kim; Sooyoung Choi; Yeong Seon Lee; Eung Chil Choi; Sunghoon Kim

doi:10.1093/jac/dkg140

Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital

Hee Jeong Yun, Sang Won Lee, Gyu Man Yoon, Su Yeon Kim, Sooyoung Choi, Yeong Seon Lee, Eung Chil Choi, Sunghoon Kim

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

82 Citations (Scopus)

Abstract

Mupirocin has been used against Gram-positive pathogenic bacteria, and is a specific inhibitor of bacterial isoleucyl-tRNA synthetase. In this work, we have determined the prevalence of mupirocin resistance among staphylococci isolated from a Korean hospital, and have investigated the characteristics of the resistance. In Staphylococcus aureus, the prevalence of high-level mupirocin resistance was 5% (16 of 319), whereas low-level mupirocin resistance was not detected. In coagulase-negative staphylococci (CoNS) the rates of high- and low-level mupirocin resistance were 16.7% (34 of 204) and 10.3% (21 of 204), respectively. The high-level resistant strains contained the ileS-2 gene, which encodes a novel staphylococcal isoleucyl-tRNA synthetase. In contrast, all of the low-level mupirocin-resistant CoNS contained the mutation V588F, which is located near the conserved motif KMSKS, within the chromosomal staphylococcal isoleucyl-tRNA synthetase gene (ileS). In conclusion, this work describes the recent, but rapid, emergence of two different types of mupirocin-resistant staphylococci in Korea, and the sequence and mutant characterization of the isoleucyl-tRNA synthetase of CoNS.

Original language	English
Pages (from-to)	619-623
Number of pages	5
Journal	Journal of Antimicrobial Chemotherapy
Volume	51
Issue number	3
DOIs	https://doi.org/10.1093/jac/dkg140
Publication status	Published - 2003 Mar 1

Bibliographical note

Funding Information:
This work was supported, in part, by a grant from the National Creative Research Initiatives, of the Ministry of Science and Technology of Korea, by the SNU Foundation & Overhead Research Fund, Seoul National University, and by the Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, to S.K., and also by a grant from the Korea Health 21 R&D Project (No. 02-PJ2-PG3-20905-0007), Ministry of Health & Welfare, Republic of Korea, to E.C.C.

All Science Journal Classification (ASJC) codes

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{3060f05c17034e94ac87cd1d37940916,

title = "Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital",

abstract = "Mupirocin has been used against Gram-positive pathogenic bacteria, and is a specific inhibitor of bacterial isoleucyl-tRNA synthetase. In this work, we have determined the prevalence of mupirocin resistance among staphylococci isolated from a Korean hospital, and have investigated the characteristics of the resistance. In Staphylococcus aureus, the prevalence of high-level mupirocin resistance was 5% (16 of 319), whereas low-level mupirocin resistance was not detected. In coagulase-negative staphylococci (CoNS) the rates of high- and low-level mupirocin resistance were 16.7% (34 of 204) and 10.3% (21 of 204), respectively. The high-level resistant strains contained the ileS-2 gene, which encodes a novel staphylococcal isoleucyl-tRNA synthetase. In contrast, all of the low-level mupirocin-resistant CoNS contained the mutation V588F, which is located near the conserved motif KMSKS, within the chromosomal staphylococcal isoleucyl-tRNA synthetase gene (ileS). In conclusion, this work describes the recent, but rapid, emergence of two different types of mupirocin-resistant staphylococci in Korea, and the sequence and mutant characterization of the isoleucyl-tRNA synthetase of CoNS.",

author = "Yun, {Hee Jeong} and Lee, {Sang Won} and Yoon, {Gyu Man} and Kim, {Su Yeon} and Sooyoung Choi and Lee, {Yeong Seon} and Choi, {Eung Chil} and Sunghoon Kim",

note = "Funding Information: This work was supported, in part, by a grant from the National Creative Research Initiatives, of the Ministry of Science and Technology of Korea, by the SNU Foundation & Overhead Research Fund, Seoul National University, and by the Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, to S.K., and also by a grant from the Korea Health 21 R&D Project (No. 02-PJ2-PG3-20905-0007), Ministry of Health & Welfare, Republic of Korea, to E.C.C.",

year = "2003",

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doi = "10.1093/jac/dkg140",

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AU - Lee, Sang Won

AU - Yoon, Gyu Man

AU - Kim, Su Yeon

AU - Choi, Sooyoung

AU - Lee, Yeong Seon

AU - Choi, Eung Chil

AU - Kim, Sunghoon

N1 - Funding Information: This work was supported, in part, by a grant from the National Creative Research Initiatives, of the Ministry of Science and Technology of Korea, by the SNU Foundation & Overhead Research Fund, Seoul National University, and by the Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, to S.K., and also by a grant from the Korea Health 21 R&D Project (No. 02-PJ2-PG3-20905-0007), Ministry of Health & Welfare, Republic of Korea, to E.C.C.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Mupirocin has been used against Gram-positive pathogenic bacteria, and is a specific inhibitor of bacterial isoleucyl-tRNA synthetase. In this work, we have determined the prevalence of mupirocin resistance among staphylococci isolated from a Korean hospital, and have investigated the characteristics of the resistance. In Staphylococcus aureus, the prevalence of high-level mupirocin resistance was 5% (16 of 319), whereas low-level mupirocin resistance was not detected. In coagulase-negative staphylococci (CoNS) the rates of high- and low-level mupirocin resistance were 16.7% (34 of 204) and 10.3% (21 of 204), respectively. The high-level resistant strains contained the ileS-2 gene, which encodes a novel staphylococcal isoleucyl-tRNA synthetase. In contrast, all of the low-level mupirocin-resistant CoNS contained the mutation V588F, which is located near the conserved motif KMSKS, within the chromosomal staphylococcal isoleucyl-tRNA synthetase gene (ileS). In conclusion, this work describes the recent, but rapid, emergence of two different types of mupirocin-resistant staphylococci in Korea, and the sequence and mutant characterization of the isoleucyl-tRNA synthetase of CoNS.

AB - Mupirocin has been used against Gram-positive pathogenic bacteria, and is a specific inhibitor of bacterial isoleucyl-tRNA synthetase. In this work, we have determined the prevalence of mupirocin resistance among staphylococci isolated from a Korean hospital, and have investigated the characteristics of the resistance. In Staphylococcus aureus, the prevalence of high-level mupirocin resistance was 5% (16 of 319), whereas low-level mupirocin resistance was not detected. In coagulase-negative staphylococci (CoNS) the rates of high- and low-level mupirocin resistance were 16.7% (34 of 204) and 10.3% (21 of 204), respectively. The high-level resistant strains contained the ileS-2 gene, which encodes a novel staphylococcal isoleucyl-tRNA synthetase. In contrast, all of the low-level mupirocin-resistant CoNS contained the mutation V588F, which is located near the conserved motif KMSKS, within the chromosomal staphylococcal isoleucyl-tRNA synthetase gene (ileS). In conclusion, this work describes the recent, but rapid, emergence of two different types of mupirocin-resistant staphylococci in Korea, and the sequence and mutant characterization of the isoleucyl-tRNA synthetase of CoNS.

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

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ER -

Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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