Preparation of peptide-loaded polymer microparticles using supercritical carbon dioxide

In Il Jung, Seoungjoo Haam, Giobin Lim, Jong Hoon Ryu

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


In this study, peptide-loaded microparticles were prepared using an aerosol solvent extraction system (ASES) by employing supercritical carbon dioxide as an antisolvent. The effects of the molecular weight of poly(Llactide) (PLLA), poly(ethylene glycol) (PEG), the blocklength of methoxy poly(ethylene glycol)-b-poly(L-lactide) (mPEG-PLLA), the blending of PLLA and PEG, and the drug-to-polymer feed ratio on the formation of leuprolideacetate (LA)-loaded microparticles and their release characteristics were investigated. Scanning electron microscope observations showed that the LA-loaded polymer particles had a spherical morphology with a smooth surface. The entrapment efficiency of LA in the ASES-processed microparticles was found to be extremely high (about 99%), whereas the initial release rate of the LA-loaded microparticles was very low for PLLA. The release rate of LA was observed to increase as the PEG block length of mPEG-PLLA and/or the drug content in the microparticles increased. When PLLA was blended with PEG, the release rate of LA from the PLLA/PEG microparticles was significantly faster compared with the correspondingmPEG-PLLA copolymer

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalBiotechnology and Bioprocess Engineering
Issue number1
Publication statusPublished - 2012 Feb

Bibliographical note

Funding Information:
work was supported by

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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