Preparation of High-quality Glabridin Extract from Glycyrrhiza glabra

Jin Hee Hong, In I.L. Jung, Yun Kyoung Cho, Seungjoo Haam, Sang Yun Lee, Giobin Lim, Jong Hoon Ryu

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7 Citations (Scopus)


Supercritical fluid extraction (SFE) using cosolvent-modified supercritical carbon dioxide and a two-step separation/purification method was investigated as a way to improve the purity of glabridin, one of the many bioactive components of Glycyrrhiza glabra (licorice). The SFE parameters were optimized using an analytical-scale SFE system in the temperature range 40–80°C and the pressure range 10–50 MPa. The extraction was then scaled up by 100 times using a preparative SFE system under the following set of optimized conditions: 40°C, 30 MPa, and SCCO2 modified with ethanol equivalent to its 25% (v/v) concentration. The glabridin purity obtained through the scaled-up SFE system was 6.2%, a much higher level than that obtained through organic solvent extraction. The licorice extract obtained by scaling up the SFE system was isolated and purified by applying alcohol precipitation/ filtration and adsorption chromatography with 80% aqueous ethanol to obtain a purer product. It was confirmed that the glabridin purity of the final extract product was increased by up to 37%, without significant loss of glabridin after two separation/purification steps.

Original languageEnglish
Pages (from-to)666-674
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 2019 Aug 1

Bibliographical note

Funding Information:
The paper was supported by The University of Suwon in 2015.

Publisher Copyright:
© 2019, The Korean Society for Biotechnology and Bioengineering and Springer.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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