Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

Joon Taek Oh; Jung Hyun Kim

doi:10.1016/S0141-0229(00)00232-5

Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

Joon Taek Oh, Jung Hyun Kim

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, K_m and V_max, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.

Original language	English
Pages (from-to)	356-361
Number of pages	6
Journal	Enzyme and Microbial Technology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/S0141-0229(00)00232-5
Publication status	Published - 2000 Sept 1

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/S0141-0229(00)00232-5

Cite this

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title = "Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres",

abstract = "Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.",

keywords = "Amyloglucosidase, Enzyme immobilization, Hydrolysis of dextrin, PNaSS microsphere, PS",

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T1 - Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

AU - Oh, Joon Taek

AU - Kim, Jung Hyun

PY - 2000/9/1

Y1 - 2000/9/1

N2 - Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.

AB - Nonporous polystyrene/poly(sodium styrene sulfonate) (PS/PNaSS) microspheres were used for immobilization of amyloglucosidase and the properties of immobilized enzyme was studied and compared with those of free enzyme. Sulfonated groups on the PS/PNaSS microspheres present a very simple, mild, and time-saving process for enzyme immobilization. Nonporous microspheres provide their surface for immobilization of enzyme and prevent the diffusion limitation problem in the pore. Despite the high concentration of bound enzyme the influence of immobilization on kinematic parameters, Km and Vmax, is relatively low compare to other porous supports. Simple and time-saving immobilization procedure as well as the effects of pH and temperature on immobilized enzyme also showed that the PS/PNaSS microspheres could be good support.

KW - Amyloglucosidase

KW - Enzyme immobilization

KW - Hydrolysis of dextrin

KW - PNaSS microsphere

KW - PS

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Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres

Abstract

All Science Journal Classification (ASJC) codes

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